Functional cardiovascular action of L-cysteine microinjected into pressor sites of the rostral ventrolateral medulla of the rat

被引:9
作者
Takemoto, Yumi [1 ]
机构
[1] Hiroshima Univ, Inst Biomed & Hlth Sci, Minami Ku, Hiroshima 7348551, Japan
关键词
L-Cysteine; Arterial blood pressure; Heart rate; RVLM; Rats; Ionotropic excitatory amino acid receptors; DEPOLARIZATION-INDUCED RELEASE; L-PROLINE; AMINO-ACIDS; GLUTAMATE; RESPONSES; ORGANIZATION; GLUTATHIONE; ASPARTATE; NEURONS; REFLEX;
D O I
10.1007/s00726-013-1651-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The endogenous sulfur-containing amino acid l-cysteine injected into the cerebrospinal fluid space of the cisterna magna increases arterial blood pressure (ABP) and heart rate (HR) in the freely moving rat. The present study examined (1) cardiovascular responses to l-cysteine microinjected into the rostral ventrolateral medulla (RVLM), where a group of neurons regulate activities of cardiovascular sympathetic neurons and (2) involvement of ionotropic excitatory amino acid (iEAA) receptors in response. In the RVLM of urethane-anesthetized rats accessed ventrally and identified with pressor responses to l-glutamate (10 mM, 34 nl), microinjections of l-cysteine increased ABP and HR dose dependently (3-100 mM, 34 nl). The cardiovascular responses to l-cysteine (30 mM) were not attenuated by a prior injection of either antagonist alone, MK801 (20 mM, 68 nl) for the NMDA type of iEAA receptors, or CNQX (2 mM) for the non-NMDA type. However, inhibition of both NMDA and non-NMDA receptors with additional prior injection of either antagonist completely blocked those responses to l-cysteine. The results indicate that l-cysteine has functional cardiovascular action in the RVLM of the anesthetized rat, and the responses to l-cysteine involve both NMDA and non-NMDA receptors albeit in a mutually exclusive parallel fashion. The findings may suggest endogenous roles of l-cysteine indirectly via iEAA receptors in the neuronal network of the RVLM for cardiovascular regulation in physiological and pathological situations.
引用
收藏
页码:863 / 872
页数:10
相关论文
共 38 条
[1]   Neuroadaptations in cystine-glutamate exchange underlie cocaine relapse [J].
Baker, DA ;
McFarland, K ;
Lake, RW ;
Shen, H ;
Tang, XC ;
Toda, S ;
Kalivas, PW .
NATURE NEUROSCIENCE, 2003, 6 (07) :743-749
[2]   Cardiac sympathetic premotor neurons [J].
Campos, RR ;
McAllen, RM .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1997, 272 (02) :R615-R620
[3]  
CURTIS DR, 1963, J PHYSIOL-LONDON, V166, P1
[4]   FUNCTIONAL-ORGANIZATION OF CENTRAL PATHWAYS REGULATING THE CARDIOVASCULAR-SYSTEM [J].
DAMPNEY, RAL .
PHYSIOLOGICAL REVIEWS, 1994, 74 (02) :323-364
[5]  
Davson H., 1987, PHYS PATHO PHYS CERE
[6]   The sympathetic control of blood pressure [J].
Guyenet, PG .
NATURE REVIEWS NEUROSCIENCE, 2006, 7 (05) :335-346
[7]   Structure of the blood-brain barrier and its role in the transport of amino acids [J].
Hawkins, RA ;
O'Kane, RL ;
Simpson, IA ;
Viña, JR .
JOURNAL OF NUTRITION, 2006, 136 (01) :218S-226S
[8]   Mechanisms of L-cysteine neurotoxicity [J].
Janáky, R ;
Varga, V ;
Hermann, A ;
Saransaari, P ;
Oja, SS .
NEUROCHEMICAL RESEARCH, 2000, 25 (9-10) :1397-1405
[9]   CYSTEINE - DEPOLARIZATION-INDUCED RELEASE FROM RAT-BRAIN INVITRO [J].
KELLER, HJ ;
DO, KQ ;
ZOLLINGER, M ;
WINTERHALTER, KH ;
CUENOD, M .
JOURNAL OF NEUROCHEMISTRY, 1989, 52 (06) :1801-1806
[10]   ROLE OF ROSTRAL VENTROLATERAL MEDULLA IN CENTRALLY MEDIATED PRESSER RESPONSES [J].
KIELY, JM ;
GORDON, FJ .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1994, 267 (04) :H1549-H1556