Influence of hormone replacement therapy on eccentric exercise induced myogenic gene expression in postmenopausal women

被引:65
作者
Dieli-Conwright, Christina M. [1 ]
Spektor, Tanya M. [2 ]
Rice, Judd C. [2 ]
Sattler, Fred R. [1 ,3 ]
Schroeder, E. Todd [1 ,3 ]
机构
[1] Univ So Calif, Keck Sch Med, Clin Exercise Res Ctr, Div Biokinesiol & Phys Therapy, Los Angeles, CA 90033 USA
[2] Univ So Calif, Keck Sch Med, USC Norris Comprehens Canc Ctr, Dept Biochem & Mol Biol, Los Angeles, CA 90033 USA
[3] Univ So Calif, Keck Sch Med, Dept Med, Los Angeles, CA 90033 USA
关键词
follistatin; myostatin; myogenic differentiation factor; HUMAN SKELETAL-MUSCLE; SATELLITE CELL ACTIVATION; MESSENGER-RNA EXPRESSION; ESTROGEN-RECEPTOR-BETA; RESISTANCE EXERCISE; HOUSEKEEPING GENE; TIME-COURSE; REAL-TIME; RT-PCR; MYOSTATIN;
D O I
10.1152/japplphysiol.00590.2009
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Dieli-Conwright CM, Spektor TM, Rice JC, Sattler FR, Schroeder ET. Influence of hormone replacement therapy on eccentric exercise induced myogenic gene expression in postmenopausal women. J Appl Physiol 107: 1381-1388, 2009. First published August 20, 2009; doi: 10.1152/japplphysiol.00590.2009.-Hormone replacement therapy (HRT) is used in postmenopausal women to relieve symptoms of menopause and prevent osteoporosis. We sought to evaluate changes in mRNA expression of key myogenic factors in postmenopausal women taking and not taking HRT following a high-intensity eccentric resistance exercise. Fourteen postmenopausal women were studied and included 6 control women not using HRT (59 +/- 4 years, 63 +/- 17 kg) and 8 women using traditional HRT (59 +/- 4 yr, 89 +/- 24 kg). Both groups performed 10 sets of 10 maximal eccentric repetitions of single-leg extension on a Cybex dynamometer at 60 degrees/s. Muscle biopsies of the vastus lateralis were obtained from the exercised leg at baseline and 4 h after the exercise bout. Gene expression was determined using RT-PCR for follistatin, forkhead box 3A (FOXO3A), muscle atrophy F-box (MAFbx), muscle ring finger-1 (MuRF-1), myogenic differentiation factor (MyoD), myogenin, myostatin, myogenic factor 5 (Myf5), and muscle regulatory factor 4 (MRF4). At rest, the HRT group expressed higher levels of MyoD, myogenin, Myf5, MRF4, and follistatin (P < 0.05). In response to eccentric exercise, follistatin, MyoD, myogenin, Myf5, and MRF4 were significantly increased (P <= 0.05) and FOXO3A, MAFbx, MuRF-1, and myostatin were significantly decreased in the control and HRT groups (P <= 0.05). Significantly greater changes in mRNA expression of follistatin, FOXO3A, MAFbx, MuRF-1, MyoD, myogenin, myostatin, Myf5, and MRF4 (p <= 0.05) occurred in the HRT group than in the control group after exercise. These data suggest that postmenopausal women using HRT express higher myogenic regulatory factor gene expression, which may reflect an attempt to preserve muscle mass. Furthermore, postmenopausal women using HRT experienced a greater myogenic response to maximal eccentric exercise.
引用
收藏
页码:1381 / 1388
页数:8
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