Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.

被引：11

作者：

Mariot, Roberta Fogliatto ^{[1
]}

de Oliveira, Luisa Abruzzi ^{[2
]}

Voorhuijzen, Marleen M. ^{[3
]}

Staats, Martijn ^{[3
]}

Hutten, Ronald C. B. ^{[4
]}

van Dijk, Jeroen P. ^{[3
]}

Kok, Esther J. ^{[3
]}

Frazzon, Jeverson ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Food Sci Inst, BR-90050170 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Dept Cellular & Mol Biol, BR-90050170 Porto Alegre, RS, Brazil

[3] Wageningen Univ, RIKILT Food Safety Inst, NL-6708 PB Wageningen, Netherlands

[4] Wageningen UR, Plant Breeding, NL-6708 PB Wageningen, Netherlands

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2016年 / 64卷 / 04期

关键词：

Solanum tuberosum; glycoalkaloids; GAMES; SGT1 and SGT3 genes; gene expression; POTATO-TUBERS; SOMATIC HYBRIDS; TRANSGENIC POTATO; ALPHA-CHACONINE; RESISTANCE; PLANT; IDENTIFICATION; EXPRESSION; BREVIDENS; ALKALOIDS;

D O I：

10.1021/acs.jafc.5b05519

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to a-chaconine and a-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.

引用

页码：988 / 996

页数：9

共 43 条

[1] FERTILE INTERSPECIFIC SOMATIC HYBRIDS OF SOLANUM - A NOVEL SOURCE OF RESISTANCE TO ERWINIA SOFT ROT [J].

AUSTIN, S ;

LOJKOWSKA, E ;

EHLENFELDT, MK ;

KELMAN, A ;

HELGESON, JP .

PHYTOPATHOLOGY, 1988, 78 (09) :1216-1220

[2]

Bejarano L, 2000, J SCI FOOD AGR, V80, P2096, DOI 10.1002/1097-0010(200011)80:14<2096::AID-JSFA757>3.0.CO

[3]

2-6

[4] REGULATION OF GLYCOALKALOID ACCUMULATION IN POTATO-TUBER DISKS [J].

BERGENSTRAHLE, A ;

TILLBERG, E ;

JONSSON, L .

JOURNAL OF PLANT PHYSIOLOGY, 1992, 140 (03) :269-275

[5] EFFECT OF POTATO GLYCOALKALOIDS, ALPHA-CHACONINE AND ALPHA-SOLANINE, ON MEMBRANE-POTENTIAL OF FROG EMBRYOS [J].

BLANKEMEYER, JT ;

STRINGER, BK ;

RAYBURN, JR ;

BANTLE, JA ;

FRIEDMAN, M .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (10) :2022-2025

[6] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[7] PlantPAN: Plant promoter analysis navigator, for identifying combinatorial cis-regulatory elements with distance constraint in plant gene groups [J].

Chang, Wen-Chi ;

Lee, Tzong-Yi ;

Huang, Hsien-Da ;

Huang, His-Yuan ;

Pan, Rong-Long .

BMC GENOMICS, 2008, 9 (1)

[8] LIPID-DERIVED SIGNALS THAT DISCRIMINATE WOUND-RESPONSIVE AND PATHOGEN-RESPONSIVE ISOPRENOID PATHWAYS IN PLANTS - METHYL JASMONATE AND THE FUNGAL ELICITOR ARACHIDONIC-ACID INDUCE DIFFERENT 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE GENES AND ANTIMICROBIAL ISOPRENOIDS IN SOLANUM-TUBEROSUM L [J].

CHOI, D ;

BOSTOCK, RM ;

AVDIUSHKO, S ;

HILDEBRAND, DF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2329-2333

[9] GLYCOALKALOID INCREASE IN SOLANUM-TUBEROSUM ON EXPOSURE TO LIGHT [J].

DALE, MFB ;

GRIFFITHS, DW ;

BAIN, H ;

TODD, D .

ANNALS OF APPLIED BIOLOGY, 1993, 123 (02) :411-418

[10] Potato glycoalkaloid impairment of fungal development [J].

Fewell, AM ;

Roddick, JG .

MYCOLOGICAL RESEARCH, 1997, 101 :597-603

← 1 2 3 4 5 →