Noninvasive in vivo imaging of CD4 cells in simian-human immunodeficiency virus (SHIV)-infected nonhuman primates

被引：48

作者：

Di Mascio, Michele ^{[1
]}

Paik, Chang H. ^{[2
]}

Carrasquillo, Jorge A. ^{[3
]}

Maeng, Jin-Soo ^{[2
]}

Jang, Beom-Su ^{[2
]}

Shin, In Soo ^{[2
]}

Srinivasula, Sharat ^{[4
]}

Byrum, Russ ^{[5
]}

Neria, Achilles ^{[2
]}

Kopp, William ^{[6
]}

Catalfamo, Marta ^{[1
]}

Nishimura, Yoshiaki ^{[1
]}

Reimann, Keith ^{[7
]}

Martin, Malcolm ^{[1
]}

Lane, H. Clifford ^{[1
]}

机构：

[1] NIAID, NIH, Bethesda, MD 20817 USA

[2] NIH, Warren G Magnuson Clin Ctr, Bethesda, MD 20892 USA

[3] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA

[4] NCI, SAIC Frederick Inc, Biostat Res Branch, Frederick, MD USA

[5] Bioqual, Rockville, MD USA

[6] NCI, Clin Serv Program, Frederick, MD 21701 USA

[7] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA

来源：

BLOOD | 2009年 / 114卷 / 02期

关键词：

MONOCLONAL-ANTIBODIES; HIV-1; INFECTION; BLOOD VOLUMES; LYMPH-NODES; T-CELLS; B-CELL; LYMPHOCYTES; DISEASE; RETENTION; THERAPY;

D O I：

10.1182/blood-2008-12-192203

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Since the earliest days of the HIV epidemic, the number of CD4(+) T cells per unit volume of blood has been recognized as a major prognostic factor for the development of AIDS in persons with HIV infection. It has also been generally accepted that approximately 2% of total body lymphocytes circulate in the blood. In the present study, we have used a nondepleting humanized anti-CD4 monoclonal antibody labeled with the gamma emitter indium-111 to visualize the CD4(+) T-cell pool in vivo in nonhuman primates with simian HIV infection. A strong correlation was noted between radiotracer uptake in spleen, tonsil, axillary lymph nodes, and peripheral blood CD4(+) T-cell counts (rho = 0.75, 0.93, and 0.85, respectively, P < .005). The relationship between radiotracer retention in lymphoid tissues and CD4(+) T-cell counts in the circulation was governed by an exponential law. These data provide an estimate for the total number of lymphocytes in the body as being between 1.9 and 2.9 x 10(12) and suggest that the partition between peripheral blood and lymphoid tissue is between 0.3% and 0.5%. (Blood. 2009; 114: 328-337)

引用

页码：328 / 337

页数：10

共 34 条

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