Loop-Mediated Isothermal Amplification for Salmonella Detection in Food and Feed: Current Applications and Future Directions

被引:97
|
作者
Yang, Qianru [1 ]
Domesle, Kelly J. [1 ]
Ge, Beilei [1 ]
机构
[1] USDA, Div Anim & Food Microbiol, Res Off, Ctr Vet Med, 8401 Muirkirk Rd, Laurel, MD 20708 USA
关键词
LAMP; Salmonella; detection; food; feed; MOLECULAR-DETECTION SYSTEM; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; RAPID DETECTION; FOODBORNE PATHOGENS; QUANTITATIVE DETECTION; COLORIMETRIC DETECTION; PROPIDIUM MONOAZIDE; BACTERIAL PATHOGENS; SENSITIVE DETECTION;
D O I
10.1089/fpd.2018.2445
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Loop-mediated isothermal amplification (LAMP) has become a powerful alternative to polymerase chain reaction (PCR) for pathogen detection in clinical specimens and food matrices. Nontyphoidal Salmonella is a zoonotic pathogen of significant food and feed safety concern worldwide. The first study employing LAMP for the rapid detection of Salmonella was reported in 2005, 5 years after the invention of the LAMP technology in Japan. This review provides an overview of international efforts in the past decade on the development and application of Salmonella LAMP assays in a wide array of food and feed matrices. Recent progress in assay design, platform development, commercial application, and method validation is reviewed. Future perspectives toward more practical and wider applications of Salmonella LAMP assays in food and feed testing are discussed.
引用
收藏
页码:309 / 331
页数:23
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