NEDD4 controls the expression of GUCD1, a protein upregulated in proliferating liver cells

被引：27

作者：

Bellet, Marina Maria ^{[1
]}

Piobbico, Danilo ^{[1
]}

Bartoli, Daniela ^{[1
]}

Castelli, Marilena ^{[1
]}

Pieroni, Stefania ^{[1
]}

Brunacci, Cinzia ^{[1
]}

Chiacchiaretta, Martina ^{[1
]}

Del Sordo, Rachele ^{[1
]}

Fallarino, Francesca ^{[1
]}

Sidoni, Angelo ^{[1
]}

Puccetti, Paolo ^{[1
]}

Romani, Luigina ^{[1
]}

Servillo, Giuseppe ^{[1
]}

Della Fazia, Maria Agnese ^{[1
]}

机构：

[1] Univ Perugia, Dept Expt Med, I-06100 Perugia, Italy

来源：

CELL CYCLE | 2014年 / 13卷 / 12期

关键词：

partial hepatectomy; liver regeneration; proliferation; HCC; ubiquitination; EPITHELIAL NA+ CHANNEL; E3 UBIQUITIN LIGASES; REGENERATION; HOPS; TRANSCRIPTION; CAMP; PHOSPHORYLATION; ISOFORMS; FAMILY; MOUSE;

D O I：

10.4161/cc.28760

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Liver regeneration is a unique means of studying cell proliferation in vivo. Screening of a large cDNA library from regenerating liver has previously allowed us to identify and characterize a cluster of genes encoding proteins with important roles in proliferative processes. Here, by examining different rat and human tissues as well as cell lines, we characterized a highly conserved gene, guanylyl cyclase domain containing 1 (GUCD1), whose modulation occurs in liver regeneration and cell cycle progression in vitro. High-level expression of GUCD1 transcripts was observed in livers from patients with hepatocellular carcinoma. A yeast two-hybrid interaction assay, aimed at identifying any relevant interaction partners of GUCD1, revealed direct interactions with NEDD4-1 (E3 ubiquitin protein ligase neural precursor cell expressed, developmentally downregulated gene 4), resulting in control of GUCD1 stability. Thus, we have characterized expression and function of a ubiquitous protein, GUCD1, which might have a role in regulating normal and abnormal cell growth in the liver.

引用

页码：1902 / 1911

页数：10

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