Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis

被引：2

作者：

Chen, Li-Chun ^{[2
]}

Huang, Sheng-Cih ^{[1
]}

Chuankhayan, Phimonphan ^{[2
]}

Chen, Chung-Der ^{[2
,3
]}

Huang, Yen-Chieh ^{[2
]}

Jeyakanthan, Jeyaraman ^{[2
]}

Pang, Hsiao-Fang ^{[4
]}

Men, Lee-Chung ^{[4
]}

Chen, Yu-Ching ^{[1
]}

Wang, Yu-Kuo ^{[1
]}

Liu, Ming-Yih ^{[2
,4
]}

Wu, Tung-Kung ^{[1
]}

Chen, Chun-Jung ^{[2
,3
,5
]}

机构：

[1] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu 30010, Taiwan

[2] Natl Synchrotron Radiat Res Ctr, Life Sci Grp, Sci Res Div, Hsinchu 30076, Taiwan

[3] Natl Tsing Hua Univ, Dept Phys, Hsinchu 30013, Taiwan

[4] Inst Nucl Energy Res, Cellulos Ethanol Project, Tao Yuan 32546, Taiwan

[5] Natl Chung Kung Univ, Inst Biotechnol, Tainan 701, Taiwan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2009年 / 65卷

关键词：

ALDO-KETO REDUCTASE; SACCHAROMYCES-CEREVISIAE; PICHIA-STIPITIS; METABOLISM; FORMS; FERMENTATION; EXPRESSION; INTERMEDIA; XYLITOL; CLONING;

D O I：

10.1107/S1744309109008719

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Xylose reductase (XR), which requires NADPH as a co-substrate, catalyzes the reduction of D-xylose to xylitol, which is the first step in the metabolism of D-xylose. The detailed three-dimensional structure of XR will provide a better understanding of the biological significance of XR in the efficient production of xylitol from biomass. XR of molecular mass 36.6 kDa from Candida tropicalis was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data from C. tropicalis XR crystals at 2.91 angstrom resolution, the unit cell belongs to space group P3(1) or P3(2). Preliminary analysis indicated the presence of four XR molecules in the asymmetric unit, with 68.0% solvent content.

引用

页码：419 / 421

页数：3

共 25 条

[1] NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae [J].

Almeida, Joao R. M. ;

Roder, Anja ;

Modig, Tobias ;

Laadan, Boaz ;

Liden, Gunnar ;

Gorwa-Grauslund, Marie-F. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 78 (06) :939-945

[2] Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF) [J].

Almeida, Joao R. M. ;

Modig, Tobias ;

Roder, Anja ;

Liden, Gunnar ;

Gorwa-Grauslund, Marie-F .

BIOTECHNOLOGY FOR BIOFUELS, 2008, 1 (1)

[3]

BECK MJ, 1986, BIOTECHNOL BIOENG S, V17, P617

[4] Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains [J].

Bettiga, Maurizio ;

Hahn-Hagerdal, Barbel ;

Gorwa-Grauslund, Marie F. .

BIOTECHNOLOGY FOR BIOFUELS, 2008, 1 (1)

[5] CLONING THE YEAST XYLULOKINASE GENE FOR THE IMPROVEMENT OF XYLOSE FERMENTATION [J].

CHANG, SF ;

HO, NWY .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1988, 17 :313-318

[6] METABOLISM OF OBLIGATORY AEROBIC YEAST RHODOTORULA-GRACILIS .4. INDUCTION OF AN ENZYME NECESSARY FOR D-XYLOSE CATABOLISM [J].

HOFER, M ;

BETZ, A ;

KOTYK, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 252 (01) :1-&

[7]

HYVONEN L, 1982, ADV FOOD RES, V28, P373

[8] The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis [J].

Kavanagh, KL ;

Klimacek, M ;

Nidetzky, B ;

Wilson, DK .

BIOCHEMISTRY, 2002, 41 (28) :8785-8795

[9] PURIFICATION AND PARTIAL CHARACTERIZATION OF AN ALDO-KETO REDUCTASE FROM SACCHAROMYCES-CEREVISIAE [J].

KUHN, A ;

VANZYL, C ;

VANTONDER, A ;

PRIOR, BA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (04) :1580-1585

[10] Identification of an NADH-dependent 5-hydroxymethylfurfural-reducing alcohol dehydrogenase in Saccharomyces cerevisiae [J].

Laadan, Boaz ;

Almeida, Joao R. M. ;

Radstrom, Peter ;

Hahn-Hagerdal, Barbel ;

Gorwa-Grauslund, Marie .

YEAST, 2008, 25 (03) :191-198

← 1 2 3 →