Spatial co-localization of multi-enzymes by inorganic nanocrystal-protein complexes

被引:160
作者
Li, Zhixian [1 ]
Zhang, Yifei [1 ]
Su, Yechao [1 ]
Ouyang, Pingkai [2 ]
Ge, Jun [1 ]
Liu, Zheng [1 ]
机构
[1] Tsinghua Univ, Dept Chem Engn, Key Lab Ind Biocatalysis, Minist Educ, Beijing 100084, Peoples R China
[2] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 211816, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
GLUCOSE DETECTION; LIPASE NANOGEL; SINGLE-ENZYME; ORGANIC MEDIA; ONE-STEP; NANOPARTICLES; SYSTEMS; NANOSTRUCTURES; PALMITATE;
D O I
10.1039/c4cc05478d
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report a simple precipitation method for the construction of spatially co-localized multi-enzyme systems based on inorganic nanocrystal-protein complexes. A spatially controlled multi-enzyme system exhibits enhanced overall catalytic performance, allowing for sensitive detection of glucose in solution.
引用
收藏
页码:12465 / 12468
页数:4
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