Partial protection against experimental vaginal candidiasis after mucosal vaccination with heat-killed Candida albicans and the mucosal adjuvant LT(R192G)
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Cárdenas-Freytag, L
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机构:Tulane Univ, Hlth Sci Ctr, Dept Immunol & Microbiol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA
Cárdenas-Freytag, L
Steele, C
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机构:Tulane Univ, Hlth Sci Ctr, Dept Immunol & Microbiol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA
Steele, C
Wormley, FL
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机构:Tulane Univ, Hlth Sci Ctr, Dept Immunol & Microbiol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA
Wormley, FL
Cheng, E
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机构:Tulane Univ, Hlth Sci Ctr, Dept Immunol & Microbiol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA
Cheng, E
Clements, JD
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机构:Tulane Univ, Hlth Sci Ctr, Dept Immunol & Microbiol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA
Clements, JD
Fidel, PL
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机构:Tulane Univ, Hlth Sci Ctr, Dept Immunol & Microbiol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA
Fidel, PL
机构:
[1] Tulane Univ, Hlth Sci Ctr, Dept Immunol & Microbiol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA
[2] Louisiana State Univ, Ctr Hlth Sci, Dept Microbiol Immunol & Parasitol, New Orleans, LA 70112 USA
The effectiveness of a mucosal vaccine composed of heat-killed Candida albicans (HK-CA) or C. albicans culture filtrate (CaCF) in conjunction with the mucosal adjuvant LT(R192G) against vulvovaginal candidiasis was examined in an estrogen-dependent murine model. Mice vaccinated intranasally with HK-CA + LT(R192G) exhibited a significant but short-lived protection accompanied by a vigorous delayed-type hypersensitivity response as well as high titers of circulating C. albicans-specific antibodies. Surprisingly, the levels of antigen-specific antibodies in the vaginal secretions of protected mice were negligible and no correlates of vaginal-associated Type 1 or Type 2 cytokines were observed. Vaginal priming with C. albicans before vaccination did not alter the protective outcome. Immunization with Caff + LT(R192G) induced a discrete level of protection when administered intrarectally but not intranasally. These results suggest that mucosal vaccination can afford partial protection against vulvovaginal candidiasis, but the precise immune mechanisms responsible for protection are complex and as yet, not well understood.