A Cytidine Deaminase Edits C to U in Transfer RNAs in Archaea

被引：55

作者：

Randau, Lennart ^{[1
]}

Stanley, Bradford J. ^{[1
]}

Kohlway, Andrew ^{[1
]}

Mechta, Sarah ^{[1
]}

Xiong, Yong ^{[1
]}

Soll, Dieter ^{[1
,2
]}

机构：

[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[2] Yale Univ, Dept Chem, New Haven, CT 06520 USA

来源：

SCIENCE | 2009年 / 324卷 / 5927期

关键词：

CRYSTAL-STRUCTURE; ADENOSINE-DEAMINASE; FUNCTIONAL IMPLICATIONS; METHANOPYRUS-KANDLERI; ESCHERICHIA-COLI; GENETIC ELEMENTS; DOMAIN; 4-THIOURIDINE; APOBEC3G; INOSINE;

D O I：

10.1126/science.1170123

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

All canonical transfer RNAs (tRNAs) have a uridine at position 8, involved in maintaining tRNA tertiary structure. However, the hyperthermophilic archaeon Methanopyrus kandleri harbors 30 (out of 34) tRNA genes with cytidine at position 8. Here, we demonstrate C-to-U editing at this location in the tRNA's tertiary core, and present the crystal structure of a tRNA-specific cytidine deaminase, CDAT8, which has the cytidine deaminase domain linked to a tRNA-binding THUMP domain. CDAT8 is specific for C deamination at position 8, requires only the acceptor stem hairpin for activity, and belongs to a unique family within the "cytidine deaminase-like" superfamily. The presence of this C-to-U editing enzyme guarantees the proper folding and functionality of all M. kandleri tRNAs.

引用

页码：657 / 659

页数：3

共 23 条

[1] THUMP - a predicted RNA-binding domain shared by 4-thiouridine, pseudouridine synthases and RNA methylases [J].

Aravind, L ;

Koonin, EV .

TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (04) :215-217

[2] RNA editing changes the identity of a mitochondrial tRNA in marsupials [J].

Borner, GV ;

Morl, M ;

Janke, A ;

Paabo, S .

EMBO JOURNAL, 1996, 15 (21) :5949-5957

[3] Structure of the DNA deaminase domain of the HIV-1 restriction factor APOBEC3G [J].

Chen, Kuan-Ming ;

Harjes, Elena ;

Gross, Phillip J. ;

Fahmy, Amr ;

Lu, Yongjian ;

Shindo, Keisuke ;

Harris, Reuben S. ;

Matsuo, Hiroshi .

NATURE, 2008, 452 (7183) :116-U16

[4] An adenosine deaminase that generates inosine at the wobble position of tRNAs [J].

Gerber, AP ;

Keller, W .

SCIENCE, 1999, 286 (5442) :1146-1149

[5] A NOVEL ENZYMATIC PATHWAY LEADING TO 1-METHYLINOSINE MODIFICATION IN HALOFERAX-VOLCANII TRANSFER-RNA [J].

GROSJEAN, H ;

CONSTANTINESCO, F ;

FOIRET, D ;

BENACHENHOU, N .

NUCLEIC ACIDS RESEARCH, 1995, 23 (21) :4312-4319

[6] Crystal structure of the anti-viral APOBEC3G catalytic domain and functional implications [J].

Holden, Lauren G. ;

Prochnow, Courtney ;

Chang, Y. Paul ;

Bransteitter, Ronda ;

Chelico, Linda ;

Sen, Udayaditya ;

Stevens, Raymond C. ;

Goodman, Myron F. ;

Chen, Xiaojiang S. .

NATURE, 2008, 456 (7218) :121-U13

[7] A Disease-causing Point Mutation in Human Mitochondrial tRNAMet Results in tRNA Misfolding Leading to Defects in Translational Initiation and Elongation [J].

Jones, Christie N. ;

Jones, Christopher I. ;

Graham, William D. ;

Agris, Paul F. ;

Spremulli, Linda L. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (49) :34445-34456

[8] Substrate specificity for 4-thiouridine modification in Escherichia coli [J].

Lauhon, CT ;

Erwin, WM ;

Ton, GN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (22) :23022-23029

[9] Identification and characterization of a human tRNA-specific adenosine deaminase related to the ADAR family of pre-mRNA editing enzymes [J].

Maas, S ;

Gerber, AP ;

Rich, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (16) :8895-8900

[10] TRANSFER-RNA GENES - LANDMARKS FOR INTEGRATION OF MOBILE GENETIC ELEMENTS IN DICTYOSTELIUM-DISCOIDEUM [J].

MARSCHALEK, R ;

BRECHNER, T ;

AMONBOHM, E ;

DINGERMANN, T .

SCIENCE, 1989, 244 (4911) :1493-1496

← 1 2 3 →