2-Cys peroxiredoxin responds to low temperature and other cues in Caragana jubata, a plant species of cold desert of Himalaya

被引：5

作者：

Bhardwaj, Pardeep Kumar ^{[1
]}

Mala, Deep ^{[1
]}

Kumar, Sanjay ^{[1
]}

机构：

[1] CSIR, Inst Himalayan Bioresource Technol, Div Biotechnol, Palampur 176061, Himachal Prades, India

来源：

MOLECULAR BIOLOGY REPORTS | 2014年 / 41卷 / 05期

关键词：

ABA; Differential display; Drought; Methyl jasmonate; RACE; Salicylic acid; CHLOROPLAST 2-CYS PEROXIREDOXIN; MOLECULAR CHARACTERIZATION; EXPRESSION ANALYSIS; ABIOTIC STRESS; ABSCISIC-ACID; GENE; PHYTOHORMONES; PROTEIN; PHOTOSYNTHESIS; METABOLISM;

D O I：

10.1007/s11033-014-3151-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 2-Cys peroxiredoxin cDNA (CjPrx) was isolated and characterized from Caragana jubata, a temperate/alpine plant species of high altitude cold desert of Himalaya and Eurasia. The cDNA obtained was 1,064 bp long consisting of an open reading frame of 789 bp encoding 262 amino acids. The calculated molecular mass of the mature protein was 28.88 kDa and pI was 5.84. Deduced amino acid sequence of CjPrx shared a high degree homology with 2-CysPrx proteins from other plants. CjPrx had both the PRX_type 2-Cys domain and thioredoxin-like superfamily domains. CjPrx contained 26.72 % alpha-helices, 6.87 % beta-turns, 20.61 % extended strands and 45.80 % random coils, and was a hydrophilic protein. Expression of CjPrx was modulated by low temperature, methyl jasmonate (MJ), salicylic acid and drought stress, but no significant change was observed in response to abscisic acid treatment. Among all the treatments, a strong up-regulation of CjPrx was observed in response to MJ treatment.

引用

页码：2951 / 2961

页数：11

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