Design Considerations of Iron-Based Nanoclusters for Noninvasive Tracking of Mesenchymal Stem Cell Homing

被引：84

作者：

Huang, Xinglu ^{[1
]}

Zhang, Fan ^{[1
,2
]}

Wang, Yu ^{[1
]}

Sun, Xiaolian ^{[1
]}

Choi, Ki Young ^{[1
]}

Liu, Dingbin ^{[1
]}

Choi, Jin-sil ^{[3
]}

Shin, Tae-Hyun ^{[3
]}

Cheon, Jinwoo ^{[3
]}

Niu, Gang ^{[1
]}

Chen, Xiaoyuan ^{[1
]}

机构：

[1] NIBIB, LOMIN, NIH, Bethesda, MD 20892 USA

[2] Xiamen Univ, Ctr Mol Imaging & Translat Med, State Key Lab Mol Vaccinol & Mol Diagnost, Sch Publ Hlth, Xiamen, Peoples R China

[3] Yonsei Univ, Dept Chem, Seoul 120749, South Korea

来源：

ACS NANO | 2014年 / 8卷 / 05期

基金：

美国国家卫生研究院;

关键词：

mesenchymal stem cell; homing; iron oxide nanoparticle; magnetic resonance imaging; CXCR4/SDF-1; alpha; INFARCTED MYOCARDIUM; CXCR4; EXPRESSION; MRI CONTRAST; IN-VITRO; MIGRATION; NANOPARTICLES; ENGRAFTMENT; CHEMOKINE; RECEPTOR; MICE;

D O I：

10.1021/nn4062726

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Stem-cell-based therapies have attracted considerable interest in regenerative medicine and ontological research. However, a major limitation of systemic delivery of stem cells is the low homing efficiency to the target site. Here, we report a serendipitous finding that various iron-based magnetic nanoparticles (MNPs) actively augment chemokine receptor CXCR4 expression of bone-marrow-derived mesenchymal stem cells (MSCs). On the basis of this observation, we designed an iron-based nanocluster that can effectively label MSCs, improve cell homing efficiency, and track the fate of the cells in vivo. Using this nanocluster, the labeled MSCs were accurately monitored by magnetic resonance imaging and improved the homing to both traumatic brain Injury and glioblastoma models as compared to unlabeled MSCs. Our findings provide a simple and safe method for imaging and targeted delivery of stem cells and extend the potential applications of iron-based MNPs in regenerative medicine and oncology.

引用

页码：4403 / 4414

页数：12

共 46 条

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