A new alternative insight of nanoemulsion conjugated with κ-carrageenan for wound healing study in diabetic mice: In vitro and in vivo evaluation

Astaxanthin and alpha-tocopherol have attracted great attention because of their properties for the management of wound healing in diabetics. This study aimed to investigate the wound healing study of astaxanthin and alphatocopherol with kappa-carrageenan nanoemulsion (AS-TP@KCNE) and to perform a histopathological study on streptozotocin (STZ)-induced diabetic mice. AS-TP@KCNE were also evaluated with aim of maintaining an effective and prolonged antidiabetic potential. KC@SENE and KC@USNE were obtained by spontaneous and ultrasonication emulsification methods and were characterized for stability, pH, particle size, and zeta potential by standard analysis. In vitro cytotoxicity and wound healing results demonstrated less toxicity and faster cell migration for the nanoemulsions at different concentrations. Based on the promising results, transdermal administration of AS-TP@KCNE can significantly restore the body weight, reduce fasting blood glucose levels, and improve glucose tolerance, which improved the ability to quickly heal of wounds in diabetic mice compared to control. In conclusion, the present study shows that the AS-TP@KCNE are biocompatible and possess healing properties that accelerate wound closure and exhibited better control of hyperglycemia and more superbly reversed the diabetes mellitus complications than STZ-induced diabetic mice. These results suggested that AS-TP@KCNE could be a promising platform for wound healing applications for diabetics.