Alcohol-Induced Changes in Methylation Status of Individual CpG Sites, and Serum Levels of Vasopressin and Atrial Natriuretic Peptide in Alcohol-Dependent Patients during Detoxification Treatment

被引：26
作者：
Glahn, Alexander [1 ]
Knorrenschild, Rafael Riera [2 ,3 ]
Rhein, Mathias [1 ]
Nassab, Mani Haschemi [1 ]
Groeschl, Michael [4 ]
Heberlein, Annemarie [1 ]
Muschler, Marc [1 ]
Frieling, Helge [1 ]
Bleich, Stefan [1 ]
Hillemacher, Thomas [1 ]
机构：
[1] Hannover Med Sch, Dept Psychiat Social Psychiat & Psychotherapy, Ctr Addict Res CARe, DE-30625 Hannover, Germany
[2] Bezirksklinikum Obermain, Clin Psychiat Psychotherapy & Psychosomat Med RR, Kutzenberg, Germany
[3] Bezirksklin Hochstadt, Div Subst Use Disorders, Hochstadt, Germany
[4] Celer Switzerland AG, Bioanalyt Lab, Fehraltorf, Switzerland
来源：
EUROPEAN ADDICTION RESEARCH | 2014年 / 20卷 / 03期
关键词：
Alcohol withdrawal; Atrial natriuretic peptide; Epigenetic regulation; Vasopressin; MESSENGER-RNA LEVELS; AFFECTIVE-DISORDERS; ANXIETY MODULATION; SUPRAOPTIC NUCLEUS; ADULT MALE; RAT-BRAIN; WITHDRAWAL; EXPRESSION; NEUROPEPTIDES; INVOLVEMENT;
D O I：
10.1159/000357473
中图分类号：
R194 [卫生标准、卫生检查、医药管理];
学科分类号：
摘要：
Disturbances of volume-regulating peptides like vasopressin (AVP) and atrial natriuretic peptide (ANP) have been described in early abstinent alcohol-dependent patients. In a longitudinal approach, we investigated whether changes in AVP and ANP serum levels correlated to cytosine-phosphatidyl-guanine (CpG) methylation of the respective gene promoters on days 1, 7 and 14 of alcohol withdrawal. We analyzed the blood samples of 99 patients suffering from alcohol dependence alongside age-and BMI-matched controls. Concerning AVP promoter methylation, we observed an interaction between time of measurement and CpG loci with CpG 2 showing a significant increase in methylation from day 1 to 14. Serum levels of AVP were significantly decreased in the patient group. Compared to healthy controls, promoter-related DNA methylation of the ANP promoter was significantly reduced on days 7 and 14. Moreover, we detected a significant interaction between CpG position and group. In both cases the difference was mainly observed at CpG 1. The present study shows significant changes in the methylation status of individual CpG sites of AVP and ANP. Observing respective alterations of AVP serum protein levels in alcohol-dependent patients during detoxification treatment, we consider methylation as a possible mode of regulation for these proteins during alcohol detoxification. (C) 2013 S. Karger AG, Basel
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页码：143 / 150
页数：8
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