Noninvasive In Vivo Tracking of Mesenchymal Stem Cells and Evaluation of Cell Therapeutic Effects in a Murine Model Using a Clinical 3.0 T MRI

被引：19

作者：

Drey, Florian ^{[1
]}

Choi, Yeong-Hoon ^{[1
,2
]}

Neef, Klaus ^{[1
,2
]}

Ewert, Birgit ^{[1
]}

Tenbrock, Arne ^{[1
]}

Treskes, Philipp ^{[1
,2
]}

Bovenschulte, Henning ^{[3
]}

Liakopoulos, Oliver J. ^{[1
]}

Brenkmann, Meike ^{[1
]}

Stamm, Christof ^{[4
]}

Wittwer, Thorsten ^{[1
,2
]}

Wahlers, Thorsten ^{[1
,2
]}

机构：

[1] Univ Cologne, Dept Cardiothorac Surg, D-50924 Cologne, Germany

[2] Univ Cologne, Ctr Mol Med Cologne, D-50924 Cologne, Germany

[3] Univ Cologne, Dept Radiol, D-50924 Cologne, Germany

[4] German Heart Inst Berlin, Dept Cardiothorac & Vasc Surg, Berlin, Germany

来源：

CELL TRANSPLANTATION | 2013年 / 22卷 / 11期

关键词：

Magnetic resonance imaging (MRI); Mesenchymal stem cells (MSCs); Cell tracking; Cardiac function; Myocardial infarction; BONE-MARROW-CELLS; MYOCARDIAL-INFARCTION; IRON-OXIDES; INBRED MICE; FEASIBILITY; MONONUCLEAR; SURVIVAL; STRAINS;

D O I：

10.3727/096368912X657747

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Cardiac cell therapy with mesenchymal stem cells (MSCs) represents a promising treatment approach for end-stage heart failure. However, little is known about the underlying mechanisms and the fate of the transplanted cells. The objective of the presented work is to determine the feasibility of magnetic resonance imaging (MRI) and in vivo monitoring after transplantation into infarcted mouse hearts using a clinical 3.0 T MRI device. The labeling procedure of bone marrow-derived MSCs with micron-sized paramagnetic iron oxide particles (MPIOs) did not affect the viability of the cells and their cell type-defining properties when compared to unlabeled cells. Using a clinical 3.0 T MRI scanner equipped with a dedicated small animal solenoid coil, 10(5) labeled MSCs could be detected and localized in the mouse hearts for up to 4 weeks after intramyocardial transplantation. Weekly ECG-gated scans using T1-weighted sequences were performed, and left ventricular function was assessed. Histological analysis of hearts confirmed the survival of labeled MSCs in the target area up to 4 weeks after transplantation. In conclusion, in vivo tracking of labeled MSCs using a clinical 3.0 T MRI scanner is feasible. In combination with assessment of heart function, this technology allows the monitoring of the therapeutic efficacy of regenerative therapies in a small animal model.

引用

页码：1971 / 1980

页数：10

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