Hemocyanin subunit organization of the gastropod Rapana thomasiana

被引:38
|
作者
Gebauer, W [1 ]
Stoeva, S
Voelter, W
Dainese, E
Salvato, B
Beltramini, M
Markl, J
机构
[1] Univ Mainz, Inst Zool, D-55099 Mainz, Germany
[2] Univ Tubingen, Dept Phys Biochem, Tubingen, Germany
[3] Univ Padua, Dept Biol, Padua, Italy
关键词
hemocyanin; Rapana thomasiana; subunit structure; disulfide bridge; mollusca;
D O I
10.1006/abbi.1999.1478
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
RtH1 and RtH2, the two hemocyanin isoforms of the prosobranch gastropod Rapana thomasiana, have been purified by anion-exchange chromatography and studied by SDS-PAGE and immunoelectrophoresis. Both subunit types are built up of eight functional units (FUs). Under reducing conditions subunit RtH2 splits into two fragments, RtH2-a-f and RtH2-gh, suggesting the presence of a disulfide bridge between FU2-f and FU2-g. By proteolytic cleavage of the subunits into three-, two-, and single-FU fragments, purification of fragments by HPLC, N-terminal sequencing of the peptides, and crossed-line immunoelectrophoresis, FUs-a-h of RtH2 and FU-a, FU-d, FU-e, and FU-f of RtH1 were identified and correlated to the eight-FUs pattern of immunoelectrophoresis. FU-a, FU-e, and FU-f of RtH1 and RtH2 are very closely related immunologically. RtH1 and RtH2 both correspond immunologically to KLH2, one of the two hemocyanin isoforms of the prosobranch gastropod Megathura crenulata. (C) 1999 Academic Press.
引用
收藏
页码:128 / 134
页数:7
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