Detection and quantitation of genetically modified maize (Bt-176 Transgenic maize) by applying ligation detection reaction and universal array technology

被引：24

作者：

Bordoni, R

Mezzelani, A

Consolandi, C

Frosini, A

Rizzi, E

Castiglioni, B

Salati, C

Marmiroli, N

Marchelli, R

Bernardi, LR

Battaglia, C

De Bellis, G

机构：

[1] Univ Milan, LITA, Consiglio Nazl Ric, Ist Tecnol Biomed, I-20090 Segrate, MI, Italy

[2] Univ Milan, LITA, Dipartimento Sci & Tecnol Biomed, I-20090 Segrate, MI, Italy

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 05期

关键词：

genetically modified organisms; GMOs; polymerase chain reaction; PCR; ligation detection reaction; LDR; universal array; Bt-176 transgenic maize;

D O I：

10.1021/jf034871e

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.

引用

收藏

页码：1049 / 1054

页数：6

相关论文

共 15 条

[1]

BORDONI R, IN PRESS CLIN CHEM

[2] Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction) [J].

Busti, Elena ;

Bordoni, Roberta ;

Castiglioni, Bianca ;

Monciardini, Paolo ;

Sosio, Margherita ;

Donadio, Stefano ;

Consolandi, Clarissa ;

Bernardi, Luigi Rossi ;

Battaglia, Cristina ;

De Bellis, Gianluca .

BMC MICROBIOLOGY, 2002, 2 (1) :1-12

[3] Detection of HLA polymorphisms by ligase detection reaction and a universal array format: A pilot study for low resolution genotyping [J].

Consolandi, C ;

Busti, E ;

Pera, C ;

Delfino, L ;

Ferrara, GB ;

Bordoni, R ;

Castiglioni, B ;

Bernardi, LR ;

Battaglia, C ;

De Bellis, G .

HUMAN IMMUNOLOGY, 2003, 64 (01) :168-178

[4] Detection of genetically modified maize by the polymerase chain reaction and capillary gel electrophoresis with UV detection and laser-induced fluorescence [J].

García-Cañas, V ;

González, R ;

Cifuentes, A .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (05) :1016-1021

[5] Ultrasensitive detection of genetically modified maize DNA by capillary gel electrophoresis with laser-induced fluorescence using different fluorescent intercalating dyes [J].

García-Cañas, V ;

González, R ;

Cifuentes, A .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (16) :4497-4502

[6] Universal DNA microarray method for multiplex detection of low abundance point mutations [J].

Gerry, NP ;

Witowski, NE ;

Day, J ;

Hammer, RP ;

Barany, G ;

Barany, F .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 292 (02) :251-262

[7] Detection of the starch modifying gbss-antisense construct in transgenic potatoes [J].

Hassan-Hauser, C ;

Mayer, W ;

Hortner, H .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 1998, 206 (02) :83-87

[8] SBE-TAGS: An array-based method for efficient single-nucleotide polymorphism genotyping [J].

Hirschhorn, JN ;

Sklar, P ;

Lindblad-Toh, K ;

Lim, YM ;

Ruiz-Gutierrez, M ;

Bolk, S ;

Langhorst, B ;

Schaffner, S ;

Winchester, E ;

Lander, ES .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) :12164-12169

[9] Detection of the genetic modification in heat-treated products of Bt maize by polymerase chain reaction [J].

Hupfer, C ;

Hotzel, H ;

Sachse, K ;

Engel, KH .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 1998, 206 (03) :203-207

[10]

McLaren JS, 2000, MOL DIAGN, V5, P257