α-MnO2 nanowire induces cytotoxicity of human lung fibroblasts based on a 3D organotypic culture

被引：0

作者：

Li, Yan ^{[1
]}

Li, Jinquan ^{[2
]}

Wang, Yixin ^{[3
]}

Wang, Pengcheng ^{[1
]}

Gong, Zhiyong ^{[3
]}

Yang, Wu ^{[3
]}

Yang, Xu ^{[2
]}

机构：

[1] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China

[2] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China

[3] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China

来源：

FERROELECTRICS | 2019年 / 546卷 / 01期

基金：

中国国家自然科学基金;

关键词：

MnO2; nanowires; human lung fibroblasts; nanotoxicity; 3D organotypic culture; PROLIFERATION; NANOMATERIAL; CELL; APOPTOSIS; FIBROSIS;

D O I：

10.1080/00150193.2019.1592451

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this study, we performed a 3D organotypic culture of human lung fibroblast (HLF) in a microfluidic device. This 3D HLF model could partly simulate the biological responses of human lung. alpha-MnO2 nanowires were successfully synthesized using the hydrothermal approach. Material characterization identified the existence of alpha-MnO2 nanostructure. Based on this 3D organotypic HLF model, our results demonstrated that alpha-MnO2 nanowires can induce cytotoxicity in dose-dependently. Because the accumulation of reactive oxygen species (ROS) and depletion of glutathione (GSH) are observed in the nanowire-treated cells. In addition, ROS generation induced intracellular increase of malondialdehyde (MDA), DNA damage and activated alpha-smooth muscle actin expression. Based on these results, we concluded that a mechanism for alpha-MnO2 nanowire-induced pulmary toxicity, which involves the accumulation of ROS, up-regulation of oxidative stress and inflammatory factors. This investigation may provide a fundamental insight to understand the nanotoxicity of wire-shaped nanomaterials.

引用

页码：1 / 12

页数：12

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[11] The influence of dexamethasone on the proliferation and apoptosis of pulmonary inflammatory cells in bleomycin-induced pulmonary fibrosis in rats [J].