Toxoid flocculation assay by laser light-scattering

被引：5

作者：

Iwaki, Masaaki ^{[1
]}

Honiuchi, Yoshinobu ^{[1
]}

Komiya, Takako ^{[1
]}

Fukuda, Tadashi ^{[1
]}

Arakawa, Yoshichika ^{[1
]}

Takahashi, Motohide ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Bacterial Pathogenesis & Infect Control, Tokyo 2080011, Japan

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2007年 / 318卷 / 1-2期

关键词：

vaccine; diphtheria; tetanus; quality control; antigen; antitoxin;

D O I：

10.1016/j.jim.2006.10.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Internationally accepted designations of antigen content for toxoid vaccines are provided by the WHO in Lf (limes flocculationis) units, based on the formation of antigen-antibody complexes. The current assay method for Lf determination involves observation of the complexes by eye, making the development of a more objective system highly desirable. Here we report a novel detection system using a laser light-scattering platelet aggregometer. The system was highly reproducible and more objective than the current method. Only three sets of duplicate data were sufficient for statistically significant determination of toxoid Lf by parabolic regression. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：138 / 146

页数：9

共 19 条

[1] BOWEN HE, 1953, J IMMUNOL, V71, P86
[2] BOWEN HE, 1953, J IMMUNOL, V70, P235
[3] The essential difference between the two optimum proportions flocculation ratios
Boyd, WC
Purnell, MA
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1944, 80 (04) : 289 - 298
[4] Finney DJ, 1978, STAT METHODS BIOL AS
[5] The titration of diphtheria toxin and antitoxin by flocculation methods.
Glenny, AT
Okell, CC
[J]. JOURNAL OF PATHOLOGY AND BACTERIOLOGY, 1924, 27 (02): : 187 - 200
[6] Harrison P, 2000, BRIT J HAEMATOL, V111, P733
[7] JACOBSEN C, 1979, IMMUNOLOGY, V236, P293
[8] Visually detectable resolution of intraoral dental films
Künzel, A
Scherkowski, D
Willers, R
Becker, J
[J]. DENTOMAXILLOFACIAL RADIOLOGY, 2003, 32 (06) : 385 - 389
[9] LEVINE L, 1965, J IMMUNOL, V94, P586
[10] THE QUANTITATIVE ESTIMATION OF DIPHTHERIA AND TETANUS TOXOIDS .1. THE FLOCCULATION TEST AND THE LF-UNIT
LYNG, J
BENTZON, MW
[J]. JOURNAL OF BIOLOGICAL STANDARDIZATION, 1987, 15 (01): : 27 - 37

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