Design, Synthesis and Application of Fluorescence Resonance Energy Transfer-Based Ratiometric Hydrazine Fluorescent Probe

Hydrazine (N2H4) is a highly toxic biochemical reagent with the capability of mutagenic, teratogenic and carcinogenic. For accurately monitoring the concentration of N2H4 in the environment and life, two FRET (fluorescence resonance energy transfer)-based dual-emissive ratiometric fluorescent probes (FRET-1/2) were designed and synthesized. The structures of both probes were charactered by H-1 NMR, C-13 NMR and FIRMS. The results prove that both probes exhibit good selectivity and sensitivity to N2H4 and can be used for detecting N2H4 in water samples.