Molecular Basis for Stellatic Acid Biosynthesis: A Genome Mining Approach for Discovery of Sesterterpene Synthases

被引：69

作者：

Matsuda, Yudai ^{[1
]}

Mitsuhashi, Takaaki ^{[1
]}

Quan, Zhiyang ^{[1
]}

Abe, Ikuro ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

ORGANIC LETTERS | 2015年 / 17卷 / 18期

关键词：

RAY CRYSTAL-STRUCTURE; ASPERGILLUS-VARIECOLOR; EMERICELLA-VARIECOLOR; BACILLUS-CLAUSII; IDENTIFICATION; STEREOCHEMISTRY; METABOLITES; CYCLASES; FUNGUS;

D O I：

10.1021/acs.orglett.5b02404

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The search for a new sesterterpene synthase in the genome of Emericella variecolor, which reportedly produces diverse sesterterpenoids, is described. One gene product (a chimeric protein with prenyltransferase and terpene cyclase domains) led to the synthesis of a novel tricyclic sesterterpene, stellata-2,6,19-triene (1), from DMAPP and IPP, and the hydrocarbon was further transformed into stellatic acid (2) by cytochrome P450 monooxygenase encoded by the gene adjacent to the sesterterpene synthase gene.

引用

页码：4644 / 4647

页数：4

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