TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins

被引：36

作者：

Li, Ying ^{[1
]}

Palmer, Arthur G., III ^{[1
]}

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

来源：

JOURNAL OF BIOMOLECULAR NMR | 2009年 / 45卷 / 04期

基金：

美国国家卫生研究院;

关键词：

ZZ-exchange; TROSY; Chemical exchange; Kinetic rates; Cadherin; E.COSY-TYPE MEASUREMENT; NMR-SPECTROSCOPY; MOLECULAR-WEIGHT; DYNAMICS; SYSTEMS;

D O I：

10.1007/s10858-009-9385-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of N-15 longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232-13237, 2007), by preserving the TROSY effect during the mixing period as well as the frequency encoding periods.

引用

页码：357 / 360

页数：4

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