Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells

被引:60
作者
Kim, Koung Li
Shin, In-Soon
Kim, Jeong-Min
Choi, Jin-Ho
Byun, Jonghoe
Jeon, Eun-Seok
Suh, Wonhee
Kim, Duk-Kyung
机构
[1] Pochon CHA Univ, Grad Sch Life Sci & Biotechnol, CHA Stem Cell Inst, Seoul 135907, South Korea
[2] Sungkyunkwan Univ, Sch Med, Samsung Biomed Res Inst, Samsung Med Ctr,Dept Med, Seoul 135710, South Korea
[3] Dankook Univ, Inst Nanosensor & Biotechnol, Dept Biol Mol, Seoul 140714, South Korea
关键词
angiogenesis; angiopoietin2; endothelial progenitor cell; Tie1; Tie2;
D O I
10.1016/j.cardiores.2006.08.002
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: Ischemia-dependent upregulation of angiopoietin2 (Ang2) led us to hypothesize the potentially proangiogenic Ang2-Tie2 signaling in endothelial progenitor cells (EPCs). Given the well-known vascular destabilizing action of Ang2 in mature endothelium, we investigated the yet unidentified mechanism behind cell-dependent differential activity of Ang2. Methods and results: Both in vitro and in vivo experiments showed that Ang2 promoted angiogenicity of human cord blood-derived EPCs, where Ang2 directly activated Tie2 and its related downstream signaling molecules. However, Ang2 had no such effect in fully differentiated human umbilical vein endothelial cells (HUVECs) under the same condition. Such a cell-dependent Tie2 activation by Ang2 was explained by comparing EPCs and HUVECs, where most Tie2 receptors in EPCs were found to be present unbound to Tie I, whereas those in HUVECs existed as heterocomplexes with Tiel. When Tie2 in HUVECs was prevented from forming heterocomplexes by silencing Tiel expression, they underwent rapid phosphorylation upon Ang2 treatment, as shown in EPCs. Conclusions: In contrast with its roles in mature endothelial cells, Ang2 has proangiogenic activities in EPC directly through Tie2 signaling pathway. Such a cell-dependent differential reactivity of Ang2 was for the first time found to be modulated by physical association between Tiel and Tie2, which inhibited Ang2-mediated Tie2 activation. (c) 2006 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:394 / 402
页数:9
相关论文
共 27 条
[1]   Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1 [J].
Ceradini, DJ ;
Kulkarni, AR ;
Callaghan, MJ ;
Tepper, OM ;
Bastidas, N ;
Kleinman, ME ;
Capla, JM ;
Galiano, RD ;
Levine, JP ;
Gurtner, GC .
NATURE MEDICINE, 2004, 10 (08) :858-864
[2]   Decreased number and impaired angiogenic function of endothelial progenitor cells in patients with chronic renal failure [J].
Choi, JH ;
Kim, KL ;
Huh, W ;
Kim, B ;
Byun, J ;
Suh, W ;
Sung, J ;
Jeon, ES ;
Oh, HY ;
Kim, DK .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2004, 24 (07) :1246-1252
[3]   Plasma angiopoietin-1, angiopoietin-2, and angiopoietin receptor tie-2 levels in congestive heart failure [J].
Chong, AY ;
Caine, GJ ;
Freestone, B ;
Blann, AD ;
Lip, GYH .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2004, 43 (03) :423-428
[4]   Angiopoietins have distinct modular domains essential for receptor binding, dimerization and superclustering [J].
Davis, S ;
Papadopoulos, N ;
Aldrich, TH ;
Maisonpierre, PC ;
Huang, T ;
Kovac, L ;
Xu, A ;
Leidich, R ;
Radziejewska, E ;
Rafique, A ;
Goldberg, J ;
Jain, V ;
Bailey, K ;
Karow, M ;
Fandl, J ;
Samuelsson, SJ ;
Ioffe, E ;
Rudge, JS ;
Daly, TJ ;
Radziejewski, C ;
Yancopoulos, GD .
NATURE STRUCTURAL BIOLOGY, 2003, 10 (01) :38-44
[5]   Angiopoietin-1 and angiopoietin-2 share the same binding domains in the Tie-2 receptor involving the first Ig-like loop and the epidermal growth factor-like repeats [J].
Fiedler, U ;
Krissl, T ;
Koidl, S ;
Weiss, C ;
Koblizek, T ;
Deutsch, U ;
Martiny-Baron, G ;
Marmé, D ;
Augustin, HG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (03) :1721-1727
[6]   Angiopoietin-2 is required for postnatal angiogenesis and lymphatic patterning, and only the latter role is rescued by angiopoietin-1 [J].
Gale, NW ;
Thurston, G ;
Hackett, SF ;
Renard, R ;
Wang, Q ;
McClain, J ;
Martin, C ;
Witte, C ;
Witte, MH ;
Jackson, D ;
Suri, C ;
Campochiaro, PA ;
Wiegand, SJ ;
Yancopoulos, GD .
DEVELOPMENTAL CELL, 2002, 3 (03) :411-423
[7]   Vascular endothelial growth factor and angiopoietin-1 stimulate postnatal hematopoiesis by recruitment of vasculogenic and hematopoietic stem cells [J].
Hattori, K ;
Dias, S ;
Heissig, B ;
Hackett, NR ;
Lyden, D ;
Tateno, M ;
Hicklin, DJ ;
Zhu, ZP ;
Witte, L ;
Crystal, RG ;
Moore, MAS ;
Rafii, S .
JOURNAL OF EXPERIMENTAL MEDICINE, 2001, 193 (09) :1005-1014
[8]   New model of tumor angiogenesis: dynamic balance between vessel regression and growth mediated by angiopoietins and VEGF [J].
Holash, J ;
Wiegand, SJ ;
Yancopoulos, GD .
ONCOGENE, 1999, 18 (38) :5356-5362
[9]   Tie receptors: New modulators of angiogenic and lymphangiogenic responses [J].
Jones, N ;
Iljin, K ;
Dumont, DJ ;
Alitalo, K .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2001, 2 (04) :257-267
[10]   Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization [J].
Kalka, C ;
Masuda, H ;
Takahashi, T ;
Kalka-Moll, WM ;
Silver, M ;
Kearney, M ;
Li, T ;
Isner, JM ;
Asahara, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :3422-3427