A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water

被引：83
作者：
Vicentini, Fernando Campanha [1 ,2 ]
Garcia, Livia L. C. [1 ]
Figueiredo-Filho, Luiz C. S. [1 ,3 ]
Janegitz, Bruno C. [1 ,4 ]
Fatibello-Filho, Orlando [1 ]
机构：
[1] Univ Fed Sao Carlos, Dept Chem, Rod Washington Luis Km 235,POB 676, BR-13560970 Sao Carlos, SP, Brazil
[2] Univ Fed Sao Carlos, Ctr Nat Sci, Rod Lauri Simoes Barros Km 12, BR-18290000 Buri, SP, Brazil
[3] Fed Inst Parana, Campus Paranavai,Rua Jose Felipe Tequinha 1400, BR-87703536 Paranavai, PR, Brazil
[4] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP, Brazil
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2016年 / 84卷
基金：
巴西圣保罗研究基金会;
关键词：
Tyrosinase biosensor; Gold nanoparticles; Dihexadecylphosphate; Catechol determination; Amperometry; GLASSY-CARBON ELECTRODE; FLOW-INJECTION DETERMINATION; PHENOLIC-COMPOUNDS; ELECTROCHEMICAL BIOSENSORS; POLYPHENOL OXIDASE; GLUCOSE-OXIDASE; NANOTUBES; IMMOBILIZATION; FABRICATION; FILM;
D O I：
10.1016/j.enzmictec.2015.12.004
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry in the presence of catechol. The determination of catechol was carried out by amperometry and presented a linear concentration range from 2.5 x 10(-6) to 9.5 x 10(-6) mol L-1 with a detection limit of 1.7 x 10(-7) mol L-1. The developed biosensor showed good repeatability and stability. Moreover, this novel amperometric method was successfully applied in the determination of catechol in natural water samples. The results were in agreement with a 95% confidence level for those obtained using the official spectrophotometric method. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：17 / 23
页数：7
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