Evidence for a Shared Nuclear Pore Complex Architecture That Is Conserved from the Last Common Eukaryotic Ancestor

被引:170
作者
DeGrasse, Jeffrey A. [1 ]
DuBois, Kelly N. [4 ]
Devos, Damien [5 ]
Siegel, T. Nicolai [2 ]
Sali, Andrej [6 ]
Field, Mark C. [4 ]
Rout, Michael P. [3 ]
Chait, Brian T. [1 ]
机构
[1] Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, New York, NY 10065 USA
[2] Rockefeller Univ, Mol Parasitol Lab, New York, NY 10065 USA
[3] Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA
[4] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England
[5] European Mol Biol Lab, D-69117 Heidelberg, Germany
[6] Univ Calif San Francisco, Dept Biopharmaceut Sci, San Francisco, CA 94143 USA
基金
美国国家卫生研究院; 英国惠康基金;
关键词
COILED-COIL PROTEIN; TRYPANOSOMA-BRUCEI; SCHIZOSACCHAROMYCES-POMBE; MOLECULAR ARCHITECTURE; TRANSPORT FACTORS; IMPORT SUBSTRATE; FISSION YEAST; CELL-CYCLE; NUCLEOPORINS; EVOLUTION;
D O I
10.1074/mcp.M900038-MCP200
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The nuclear pore complex (NPC) is a macromolecular assembly embedded within the nuclear envelope that mediates bidirectional exchange of material between the nucleus and cytoplasm. Our recent work on the yeast NPC has revealed a simple modularity in its architecture and suggested a common evolutionary origin of the NPC and vesicle coating complexes in a progenitor protocoatomer. However, detailed compositional and structural information is currently only available for vertebrate and yeast NPCs, which are evolutionarily closely related. Hence our understanding of NPC composition in a full evolutionary context is sparse. Moreover despite the ubiquitous nature of the NPC, sequence searches in distant taxa have identified surprisingly few NPC components, suggesting that much of the NPC may not be conserved. Thus, to gain a broad perspective on the origins and evolution of the NPC, we performed proteomics analyses of NPC-containing fractions from a divergent eukaryote (Trypanosoma brucei) and obtained a comprehensive inventory of its nucleoporins. Strikingly trypanosome nucleoporins clearly share with metazoa and yeast their fold type, domain organization, composition, and modularity. Overall these data provide conclusive evidence that the majority of NPC architecture is indeed conserved throughout the Eukaryota and was already established in the last common eukaryotic ancestor. These findings strongly support the hypothesis that NPCs share a common ancestry with vesicle coating complexes and that both were established very early in eukaryotic evolution. Molecular & Cellular Proteomics 8: 2119-2130, 2009.
引用
收藏
页码:2119 / 2130
页数:12
相关论文
共 75 条
[1]   The new higher level classification of eukaryotes with emphasis on the taxonomy of protists [J].
Adl, SM ;
Simpson, AGB ;
Farmer, MA ;
Andersen, RA ;
Anderson, OR ;
Barta, JR ;
Bowser, SS ;
Brugerolle, G ;
Fensome, RA ;
Fredericq, S ;
James, TY ;
Karpov, S ;
Kugrens, P ;
Krug, J ;
Lane, CE ;
Lewis, LA ;
Lodge, J ;
Lynn, DH ;
Mann, DG ;
McCourt, RM ;
Mendoza, L ;
Moestrup, O ;
Mozley-Standridge, SE ;
Nerad, TA ;
Shearer, CA ;
Smirnov, AV ;
Spiegel, FW ;
Taylor, MFJR .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 2005, 52 (05) :399-451
[2]   ARCHITECTURE OF THE XENOPUS NUCLEAR-PORE COMPLEX REVEALED BY 3-DIMENSIONAL CRYOELECTRON MICROSCOPY [J].
AKEY, CW ;
RADERMACHER, M .
JOURNAL OF CELL BIOLOGY, 1993, 122 (01) :1-19
[3]   The molecular architecture of the nuclear pore complex [J].
Alber, Frank ;
Dokudovskaya, Svetlana ;
Veenhoff, Liesbeth M. ;
Zhang, Wenzhu ;
Kipper, Julia ;
Devos, Damien ;
Suprapto, Adisetyantari ;
Karni-Schmidt, Orit ;
Williams, Rosemary ;
Chait, Brian T. ;
Sali, Andrej ;
Rout, Michael P. .
NATURE, 2007, 450 (7170) :695-701
[4]   Determining the architectures of macromolecular assemblies [J].
Alber, Frank ;
Dokudovskaya, Svetlana ;
Veenhoff, Liesbeth M. ;
Zhang, Wenzhu ;
Kipper, Julia ;
Devos, Damien ;
Suprapto, Adisetyantari ;
Karni-Schmidt, Orit ;
Williams, Rosemary ;
Chait, Brian T. ;
Rout, Michael P. ;
Sali, Andrej .
NATURE, 2007, 450 (7170) :683-694
[5]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[6]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[7]   Protein repeats: Structures, functions, and evolution [J].
Andrade, MA ;
Perez-Iratxeta, C ;
Ponting, CP .
JOURNAL OF STRUCTURAL BIOLOGY, 2001, 134 (2-3) :117-131
[8]   The two tempos of nuclear pore complex evolution: highly adapting proteins in an ancient frozen structure [J].
Bapteste, E ;
Charlebois, RL ;
Macleod, D ;
Brochier, C .
GENOME BIOLOGY, 2005, 6 (10)
[9]   Nuclear pore complex structure and dynamics revealed by cryoelectron tomography [J].
Beck, M ;
Förster, F ;
Ecke, M ;
Plitzko, JM ;
Melchior, F ;
Gerisch, G ;
Baumeister, W ;
Medalia, O .
SCIENCE, 2004, 306 (5700) :1387-1390
[10]   Quantitative models of nuclear transport [J].
Becskei, A ;
Mattaj, LW .
CURRENT OPINION IN CELL BIOLOGY, 2005, 17 (01) :27-34