Transferrin Decorated Thermoresponsive Nanogels as Magnetic Trap Devices for Circulating Tumor Cells

被引：27

作者：

Asadian-Birjand, Mazdak ^{[1
]}

Biglione, Catalina ^{[2
]}

Bergueiro, Julian ^{[1
]}

Cappelletti, Ariel ^{[2
]}

Rahane, Chinmay ^{[3
]}

Chate, Govind ^{[3
]}

Khandare, Jayant ^{[3
]}

Klemke, Bastian ^{[4
]}

Strumia, Miriam C. ^{[2
]}

Calderon, Marcelo ^{[1
]}

机构：

[1] Free Univ Berlin, Inst Chem & Biochem, Takustr 3, D-14195 Berlin, Germany

[2] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Quim, LAMAP Lab Mat Polimer,IMBIV CONICET, X5000HUA, RA-5000 Cordoba, Argentina

[3] MAEERs Maharashtra Inst Pharm, Pune 411038, Maharashtra, India

[4] Helmholtz Zentrum Berlin Mat & Energie GmbH, D-14109 Berlin, Germany

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2016年 / 37卷 / 05期

关键词：

cell sorting; circulating tumor cells; thermoresponsive nanogels; transferrin; CANCER-PATIENTS; DRUG-DELIVERY; PERIPHERAL-BLOOD; NANOPARTICLES; POLYGLYCEROL; TRANSITION; CAPTURE; DESIGN; LIGAND; SIZE;

D O I：

10.1002/marc.201500590

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A rational design of magnetic capturing nanodevices, based on a specific interaction with circulating tumor cells (CTCs), can advance the capturing efficiency and initiate the development of modern smart nanoformulations for rapid isolation and detection of these CTCs from the bloodstream. Therefore, the development and evaluation of magnetic nanogels (MNGs) based on magnetic nanoparticles and linear thermoresponsive polyglycerol for the capturing of CTCs with overexpressed transferrin (Tf+) receptors has been presented in this study. The MNGs are synthesized using a strain-promoted "click" approach which has allowed the in situ surface decoration with Tf-polyethylene glycol (PEG) ligands of three different PEG chain lengths as targeting ligands. An optimal value of around 30% of cells captures is achieved with a linker of eight ethylene glycol units. This study shows the potential of MNGs for the capture of CTCs and the necessity of precise control over the linkage of the targeting moiety to the capturing device.

引用

页码：439 / 445

页数：7

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