Wilms' tumor gene 1 expression analysis by real-time quantitative polymerase chain reaction for monitoring of minimal residual disease in acute leukemia

被引：21
|
作者：
Nowakowska-Kopera, Alicja [1 ]
Sacha, Tomasz [1 ]
Florek, Izabela [1 ]
Zawada, Magdalena [1 ]
Czekalska, Sylwia [1 ]
Skotnicki, Aleksander B. [1 ]
机构：
[1] Jagiellonian Univ, Dept Hematol, Coll Med, PL-31501 Krakow, Poland
来源：
LEUKEMIA & LYMPHOMA | 2009年 / 50卷 / 08期
关键词：
WT1; gene; minimal residual disease; acute leukemia; ACUTE MYELOID-LEUKEMIA; WT1; MESSENGER-RNA; RT-PCR; PROGNOSTIC-SIGNIFICANCE; SUPPRESSOR GENE; TRANSCRIPTS; QUANTIFICATION; RELAPSE; MARKER; TRANSPLANTATION;
D O I：
10.1080/10428190903050021
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Wilms' tumor gene 1 (WT1) gene expression was analyzed in 32 patient with acute myeloid leukemia (AML) and 18 with acute lymphoblastic leukemia (ALL) to investigate whether it could serve as a MRD marker. Ninety-four percent of patients with acute leukemia showed high WT1 expression at presentation. WT1 expression as a MRD marker was evaluated in 36 patients. The rise of WT1 expression preceded the hematological relapse by similar to 4 months (mean time 129 days; range 6-298). The prognostic significance of WT1 expression was analyzed in 30 patients with AML. WT1 expression higher than 20 WT1 copies /10(4) ABL copies after induction and consolidation chemotherapy was associated with shorter OS. WT1 expression analysis could be a useful tool for MRD monitoring in acute leukemia.
引用
收藏
页码：1326 / 1332
页数：7
相关论文
共 50 条
  • [41] Minimal residual disease detection in acute lymphoblastic leukemia: Real improvement with the real-time quantitative PCR method?
    Neale, GAM
    Campana, D
    Pui, CH
    JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY, 2003, 25 (02) : 100 - 102
  • [42] Clinical implications of minimal residual disease monitoring by quantitative polymerase chain reaction in acute myeloid leukemia patients bearing nucleophosmin (NPM1) mutations
    W-C Chou
    J-L Tang
    S-J Wu
    W Tsay
    M Yao
    S-Y Huang
    K-C Huang
    C-Y Chen
    C-F Huang
    H-F Tien
    Leukemia, 2007, 21 : 998 - 1004
  • [43] Clinical implications of minimal residual disease monitoring by quantitative polymerase chain reaction in acute myeloid leukemia patients bearing nucleophosmin (NPM1) mutations
    Chou, W-C
    Tang, J-L
    Wu, S-J
    Tsay, W.
    Yao, M.
    Huang, S-Y
    Huang, K-C
    Chen, C-Y
    Huang, C-F
    Tien, H-F
    LEUKEMIA, 2007, 21 (05) : 998 - 1004
  • [44] Wilms tumor 1 gene expression in acute myeloid leukemia: prognostic significance and usefulness in minimal residual disease monitoring-a case-control study
    Donia, Hanaa Mahmoud
    Elsweify, Nada Mahmoud
    Farahat, Nahla Mohamed
    Nadwan, Eman Attia
    EGYPTIAN JOURNAL OF MEDICAL HUMAN GENETICS, 2022, 23 (01)
  • [45] Improved Minimal Residual Disease Detection by Targeted Quantitative Polymerase Chain Reaction in Nucleophosmin 1 Type a Mutated Acute Myeloid Leukemia
    Pettersson, Louise
    Leveen, Per
    Axler, Olof
    Dvorakova, Dana
    Juliusson, Gunnar
    Ehinger, Mats
    GENES CHROMOSOMES & CANCER, 2016, 55 (10): : 750 - 766
  • [46] Minimal residual disease monitoring of NML-EPS15 fusion gene in infants' acute leukemia using quantitative real-time PCR assay
    Tsaur, G.
    Ivanova, A.
    Kovalev, S.
    Misurin, A.
    Suchkova, M.
    Aleinikova, O.
    Kustanovich, A.
    Yakovleva, Y.
    Saveliev, L.
    Fechina, L.
    CLINICAL CHEMISTRY, 2008, 54 (06) : A164 - A164
  • [47] Real-time quantitative of minimal residual PCR: Standardized detection disease in pediatric acute lymphoblastic leukemia
    Pine, SR
    Moy, FH
    Wiemels, JL
    Gill, RK
    Levendoglu-Tugal, O
    Ozkaynak, MF
    Sandoval, C
    Jayabose, S
    JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY, 2003, 25 (02) : 103 - 108
  • [48] Quantitative assessment of WT1 expression by real time quantitative PCR may be a useful tool for monitoring minimal residual disease in acute leukemia patients
    Cilloni, D
    Gottardi, E
    De Micheli, D
    Serra, A
    Volpe, G
    Messa, F
    Rege-Cambrin, G
    Guerrasio, A
    Divona, M
    Lo Coco, F
    Saglio, G
    LEUKEMIA, 2002, 16 (10) : 2115 - 2121
  • [49] Quantitative Assessment of Minimal Residual Disease in Childhood Lymphoid Malignancies Using an Allele-Specific Oligonucleotide Real-Time Quantitative Polymerase Chain Reaction
    Mitsu Tarusawa
    Akiko Yashima
    Mikiya Endo
    Chihaya Maesawa
    International Journal of Hematology, 2002, 75 : 166 - 173
  • [50] Quantitative assessment of WT1 expression by real time quantitative PCR may be a useful tool for monitoring minimal residual disease in acute leukemia patients
    D Cilloni
    E Gottardi
    D De Micheli
    A Serra
    G Volpe
    F Messa
    G Rege-Cambrin
    A Guerrasio
    M Divona
    F Lo Coco
    G Saglio
    Leukemia, 2002, 16 : 2115 - 2121
12345