Study of the 'Raman spectroscopic signature of life' in mitochondria isolated from budding yeast

被引：23
作者：
Chiu, Liang-da [1 ]
Ando, Masahiro [1 ]
Hamaguchi, Hiro-o [1 ]
机构：
[1] Univ Tokyo, Sch Sci, Dept Chem, Bunkyo Ku, Tokyo 1130033, Japan
来源：
JOURNAL OF RAMAN SPECTROSCOPY | 2010年 / 41卷 / 01期
关键词：
Raman spectroscopic signature of life; budding yeast; mitochondria; metabolic activity; respiration inhibition; CELLS;
D O I：
10.1002/jrs.2425
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
The 'Raman spectroscopic signature of life' is a Raman band at 1602 cm(-1) that sharply reflects the metabolic activity of cell mitochondria. Here we report the study of thissignature in isolated yeast mitochondria. The existence and behaviour of the 1602 cm(-1) band in isolated mitochondria have been confirmed to be the same as in living yeast cells: the intensity of the band decreases with timewhen a respiration inhibitor, sodium azide, is added. The present study shows the significance of isolated mitochondria in elucidating the origin of this still unassigned Raman band. Copyright (C). 2009 John Wiley & Sons, Ltd.
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页码：2 / 3
页数：2
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