Tumour necrosis factor-α but not lipopolysaccharide enhances preference of murine dendritic cells for Th2 differentiation

被引：27
作者：
Kikuchi, K [1 ]
Yanagawa, Y [1 ]
Aranami, T [1 ]
Iwabuchi, C [1 ]
Iwabuchi, K [1 ]
Onoé, K [1 ]
机构：
[1] Hokkaido Univ, Div Immunobiol, Inst Med Genet, Kita Ku, Sapporo, Hokkaido 0600815, Japan
来源：
IMMUNOLOGY | 2003年 / 108卷 / 01期
关键词：
D O I：
10.1046/j.1365-2567.2003.01537.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Using murine spleen-derived dendritic cells (DC) and DO11.10 T cells specific for ovalbumin (OVA), the influences of maturational condition and antigen dose on the capability of DC to induce helper T-cell (Th) differentiation were analysed. Immature DC (iDC) with high- or low-dose OVA(323-339) predominantly induced Th1 or Th2 responses in DO11.10 T cells, respectively. DC matured by tumour necrosis factor-alpha (TNF/DC) induced a significantly higher Th2 response in the presence of low-dose OVA(323-339) than iDC and DC matured by lipopolysaccharide (LPS) (LPS/DC). In the presence of high-dose OVA(323-339) , LPS/DC induced significantly lower levels of Th1 response than iDC. Under these conditions no difference in the Th1 response was noted between TNF/DC and iDC. The enhanced capability of TNF/DC with a low-dose antigen for Th2 polarization and the decreased preference of LPS/DC with a high-dose antigen to Th1 polarization were not related to the amount of IL-12 produced in these cultures. These results demonstrate for the first time that TNF/DC with a low-dose antigen are potent inducers of Th2 differentiation.
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页码：42 / 49
页数：8
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