A biolayer interferometry-based competitive biosensor for rapid and sensitive detection of saxitoxin

被引：57

作者：

Gao, Shunxiang ^{[1
,2
,3
,4
]}

Zheng, Xin ^{[5
]}

Wu, Jihong ^{[1
,2
,3
,4
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, Inst Brain Sci, Eye & ENT Hosp,State Key Lab Med Neurobiol, Shanghai 200032, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Collaborat Innovat Ctr Brain Sci, Shanghai 200032, Peoples R China

[3] Shanghai Key Lab Visual Impairment & Restorat, Shanghai 200032, Peoples R China

[4] Fudan Univ, Minist Hlth, Key Lab Myopia, Shanghai 200032, Peoples R China

[5] Second Mil Med Univ, Changhai Hosp, Dept Lab Diag, Shanghai 200433, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 246卷

基金：

中国国家自然科学基金;

关键词：

Saxitoxin; Aptamer; Biolayer interferometry; Biosensor; SHELLFISH POISONING TOXINS; LIQUID-CHROMATOGRAPHY; PRECHROMATOGRAPHIC OXIDATION; QUANTITATIVE-DETERMINATION; FLUORESCENCE DETECTION; ASSAY; APTAMERS; IMMUNOASSAY; MOLECULES; SELECTION;

D O I：

10.1016/j.snb.2017.02.078

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Saxitoxin is a marine neurotoxin, which is commonly found as a paralytic shellfish toxin. It is characterized by strong toxicity, rapid effects and wide distribution with significant public health impacts. The ethical issues and technical defects associated with the currently applied surveillance system for paralytic shellfish toxins are encouraging further studies to develop suitable alternatives. Here, we report a label-free and real-time optical biolayer interferometry competitive biosensor for the detection of saxitoxin using aptamers as specific receptors. The biosensor exhibited a broad detection range from 10 to 2000 ng/mL of saxitoxin (linear range from 100 to 800 ng/mL), with a low detection limit of 0.5 ng/mL. Furthermore, the biosensor showed a high degree of selectivity for saxitoxin, and good reproducibility and stability with real samples. We believe that this novel biosensor offers a promising alternative to traditional analytical methods for the sensitive and rapid (7 min) detection of saxitoxin. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：169 / 174

页数：6

共 31 条

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