Two-dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients

被引：100
作者：
Adessi, C
Miege, C
Albrieux, C
Rabilloud, T
机构：
[1] CEA, LAB CHIM PROT, GRENOBLE, FRANCE
[2] CEA, PHYSIOL CELLULAIRE VEGETALE LAB, GRENOBLE, FRANCE
[3] CEA, LAB BIOENERGET CELLULAIRE & PATHOL, GRENOBLE, FRANCE
来源：
ELECTROPHORESIS | 1997年 / 18卷 / 01期
关键词：
membrane proteins; two-dimensional polyacrylamide gel electrophoresis; immobilized pH gradients;
D O I：
10.1002/elps.1150180124
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Membrane proteins were separated by high resolution two-dimensional (2-D) electrophoresis. On isoelectric focusing (IEF) with immobilized pH gradients severe protein losses in the resulting 2-D map were observed when compared with carrier ampholyte-based IEF This has been noticed for two different biological systems, namely the chloroplast envelope of spinach and the endocytic vesicles from Dictyostelium discoideum. The possible mechanisms of these losses on immobilized pH gradients are discussed.*
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页码：127 / 135
页数：9
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