Rhamnocitrin glycosides from Oxytropis chiliophylla

Repeated chromatography of the n-BuOH soluble fraction from ethanol extract of the aerial part of Oxytropis chiliophylla led to the isolation of six new rhamnocitrin glycosides, named oxychilioflavonosides A-F (1-6), together with eight known rhamnocitrin glycosides (7-14) of which six were isolated from the genus Oxytropis for the first time. The structures of these compounds were elucidated by extensive spectroscopic techniques and chemical methods. All isolated compounds were evaluated for their anti-inflammatory activity in lipopolysaccharide (LPS)-induced BV-2 microglial cells, and compound 11 showed potent inhibition on NO production with IC50 value of 9.91 mu M. (C) 2016 Phytochemical Society of Europe. Published by Elsevier Ltd. All rights reserved.