Identification and characterization of outer membrane proteins G1a and G1b of Moraxella catarrhalis

Moraxella catarrhalis is an important cause of otitis media, sinusitis, and lower respiratory tract infections in patients with chronic obstructive pulmonary disease. The purified outer membrane of M. catarrhalis contains a 29 kDa band, previously named outer membrane protein G1 (OMP G1). Polyclonal antiserum to the OMP G1 band was used to screen a genomic lambda phage library and the gene for OMP G1a was cloned and sequenced. Analysis of outer membrane by isoelectric focusing and amino-terminal protein sequence of the 29 kDa band revealed that the band is actually two individual proteins designated OMP G1a and OMP G1b. OMP G1a is a lipoprotein with an isoelectric point of 4. OMP Gib contains an unblocked amino-terminus and has an isoelectric point of 9. Analysis of the sequence of OMP G1a and OMP Gib from 25 clinical isolates revealed a high degree of conservation among strains. The sequence conservation of OMP G1a and OMP Gib among strains, combined with previous observations that OMP G1a and OMP Gib contain epitopes on the bacterial surface, indicate that OMP G1a and OMP Gib are potential vaccine antigens for M. catarrhalis. (C) 2004 Elsevier Ltd. All rights reserved.