Expression of a modified Cry1Ie gene in E-coli and in transgenic tobacco confers resistance to corn borer

被引：17
作者：
Liu, YJ
Song, FP
He, KL
Yuan, Y
Zhang, XX
Gao, P
Wang, JH
Wang, GY [1 ]
机构：
[1] China Agr Univ, Natl Ctr Maize Improvement, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
[2] CAAS, Inst Plant Protect, State Key Lab Biol Plant Dis & Insects Pests, Beijing 100094, Peoples R China
来源：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2004年 / 36卷 / 04期
关键词：
Bacillus thuringiensis; Cry1Iem; transgenic tobacco; insect-resistance;
D O I：
10.1093/abbs/36.4.309
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The wild-type Cry1Ie gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1Ie gene (designated as Cry1Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1Iem protein had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal activity against corn borer.
引用
收藏
页码：309 / 313
页数：5
相关论文
共 33 条
  • [1] THE RECONSTRUCTION AND EXPRESSION OF A BACILLUS-THURINGIENSIS CRYIIIA GENE IN PROTOPLASTS AND POTATO PLANTS
    ADANG, MJ
    BRODY, MS
    CARDINEAU, G
    EAGAN, N
    ROUSH, RT
    SHEWMAKER, CK
    JONES, A
    OAKES, JV
    MCBRIDE, KE
    [J]. PLANT MOLECULAR BIOLOGY, 1993, 21 (06) : 1131 - 1145
  • [2] FIELD-EVALUATION OF EUROPEAN CORN-BORER CONTROL IN PROGENY OF 173 TRANSGENIC CORN EVENTS EXPRESSING AN INSECTICIDAL PROTEIN FROM BACILLUS-THURINGIENSIS
    ARMSTRONG, CL
    PARKER, GB
    PERSHING, JC
    BROWN, SM
    SANDERS, PR
    DUNCAN, DR
    STONE, T
    DEAN, DA
    DEBOER, DL
    HART, J
    HOWE, AR
    MORRISH, FM
    PAJEAU, ME
    PETERSEN, WL
    REICH, BJ
    RODRIGUEZ, R
    SANTINO, CG
    SATE, SJ
    SCHULER, W
    SIMS, SR
    STEHLING, S
    TAROCHIONE, LJ
    FROMM, ME
    [J]. CROP SCIENCE, 1995, 35 (02) : 550 - 557
  • [3] LACK OF CROSS-RESISTANCE TO OTHER BACILLUS-THURINGIENSIS CRYSTAL PROTEINS IN A POPULATION OF PLUTELLA-XYLOSTELLA HIGHLY RESISTANT TO CRYIA(B)
    BALLESTER, V
    ESCRICHE, B
    MENSUA, JL
    RIETHMACHER, GW
    FERRE, J
    [J]. BIOCONTROL SCIENCE AND TECHNOLOGY, 1994, 4 (04) : 437 - 443
  • [4] BACILLUS-THURINGIENSIS DELTA-ENDOTOXIN EXPRESSED IN TRANSGENIC NICOTIANA-TABACUM PROVIDES RESISTANCE TO LEPIDOPTERAN INSECTS
    BARTON, KA
    WHITELEY, HR
    YANG, NS
    [J]. PLANT PHYSIOLOGY, 1987, 85 (04) : 1103 - 1109
  • [5] Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize
    Bohorova, N
    Frutos, R
    Royer, M
    Estañol, P
    Pacheco, M
    Rascón, Q
    McLean, S
    Hoisington, D
    [J]. THEORETICAL AND APPLIED GENETICS, 2001, 103 (6-7) : 817 - 826
  • [6] Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants
    Christensen, AH
    Quail, PH
    [J]. TRANSGENIC RESEARCH, 1996, 5 (03) : 213 - 218
  • [7] MESSENGER-RNA TRANSCRIPTS OF SEVERAL PLANT GENES ARE POLYADENYLATED AT MULTIPLE SITES INVIVO
    DEAN, C
    TAMAKI, S
    DUNSMUIR, P
    FAVREAU, M
    KATAYAMA, C
    DOONER, H
    BEDBROOK, J
    [J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (05) : 2229 - 2240
  • [8] Doyle JJ., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362
  • [9] INSECT TOLERANT TRANSGENIC TOMATO PLANTS
    FISCHHOFF, DA
    BOWDISH, KS
    PERLAK, FJ
    MARRONE, PG
    MCCORMICK, SM
    NIEDERMEYER, JG
    DEAN, DA
    KUSANOKRETZMER, K
    MAYER, EJ
    ROCHESTER, DE
    ROGERS, SG
    FRALEY, RT
    [J]. BIO-TECHNOLOGY, 1987, 5 (08): : 807 - 813
  • [10] Managing insect resistance to plants producing Bacillus thuringiensis toxins
    Frutos, R
    Rang, C
    Royer, F
    [J]. CRITICAL REVIEWS IN BIOTECHNOLOGY, 1999, 19 (03) : 227 - 276
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