Label-Free Free-Solution Single-Molecule Protein Small Molecule Interaction Observed by Double-Nanohole Plasmonic Trapping

被引：68

作者：

Al Balushi, Ahmed A. ^{[1
]}

Gordon, Reuven ^{[1
]}

机构：

[1] Univ Victoria, Dept Elect Engn, Victoria, BC V8W 3P6, Canada

来源：

ACS PHOTONICS | 2014年 / 1卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

double nanohole; optical trapping; nanoparticles; nanoplasmonics; subwavelength apertures; NANOPARTICLES; SPECTROSCOPY; DYNAMICS; BINDING;

D O I：

10.1021/ph5000314

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The interaction of proteins with small molecules is fundamental to their 100 function in living organisms, and it is widely studied in drug development. Here we use the double nanohole optical trapping technique to observe real-time label-free free-solution single-molecule dynamics of three complexes: biotin streptavidin, biotin monovalent streptavidin, and acetylsalicylic acid cyclooxygenase 2. Radically different behavior is seen between the protein with and without the small molecule binding. This detection platform is scalable, inexpensive, and highly sensitive, which may transform drug discovery based on protein small molecule interactions.

引用

页码：389 / 393

页数：5

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