Label-Free Free-Solution Single-Molecule Protein Small Molecule Interaction Observed by Double-Nanohole Plasmonic Trapping

被引：68

作者：

Al Balushi, Ahmed A. ^{[1
]}

Gordon, Reuven ^{[1
]}

机构：

[1] Univ Victoria, Dept Elect Engn, Victoria, BC V8W 3P6, Canada

来源：

ACS PHOTONICS | 2014年 / 1卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

double nanohole; optical trapping; nanoparticles; nanoplasmonics; subwavelength apertures; NANOPARTICLES; SPECTROSCOPY; DYNAMICS; BINDING;

D O I：

10.1021/ph5000314

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The interaction of proteins with small molecules is fundamental to their 100 function in living organisms, and it is widely studied in drug development. Here we use the double nanohole optical trapping technique to observe real-time label-free free-solution single-molecule dynamics of three complexes: biotin streptavidin, biotin monovalent streptavidin, and acetylsalicylic acid cyclooxygenase 2. Radically different behavior is seen between the protein with and without the small molecule binding. This detection platform is scalable, inexpensive, and highly sensitive, which may transform drug discovery based on protein small molecule interactions.

引用

页码：389 / 393

页数：5

共 28 条

[1] Observing single protein binding by optical transmission through a double nanohole aperture in a metal film [J].

Al Balushi, Ahmed A. ;

Zehtabi-Oskuie, Ana ;

Gordon, Reuven .

BIOMEDICAL OPTICS EXPRESS, 2013, 4 (09) :1504-1511

[2] Small-molecule inhibitors of protein-protein interactions: Progressing towards the dream [J].

Arkin, MR ;

Wells, JA .

NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (04) :301-317

[3] Free-solution, label-free molecular interactions studied by back-scattering interferometry [J].

Bornhop, Darryl J. ;

Latham, Joey C. ;

Kussrow, Amanda ;

Markov, Dmitry A. ;

Jones, Richard D. ;

Sorensen, Henrik S. .

SCIENCE, 2007, 317 (5845) :1732-1736

[4]

Box G.E.P., 1979, Time Series Analysis: Forecasting and Control

[5] Dynamics of the Streptavidin-Biotin Complex in Solution and in Its Crystal Lattice: Distinct Behavior Revealed by Molecular Simulations [J].

Cerutti, David S. ;

Le Trong, Isolde ;

Stenkamp, Ronald E. ;

Lybrand, Terry P. .

JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (19) :6971-6985

[6] Single-molecule Spectroscopy using nanoporous membranes [J].

Chansin, Guillaume A. T. ;

Mulero, Rafael ;

Hong, Jongin ;

Kim, Min Jun ;

deMello, Andrew J. ;

Edel, Joshua B. .

NANO LETTERS, 2007, 7 (09) :2901-2906

[7] Enhanced Optical Trapping and Arrangement of Nano-Objects in a Plasmonic Nanocavity [J].

Chen, Chang ;

Juan, Mathieu L. ;

Li, Yi ;

Maes, Guido ;

Borghs, Gustaaf ;

Van Dorpe, Pol ;

Quidant, Romain .

NANO LETTERS, 2012, 12 (01) :125-132

[8]

Cizmar T., 2010, J PHYS B ATOM MOL PH, V34

[9] Dissociation Kinetics of the Streptavidin-Biotin Interaction Measured Using Direct Electrospray Ionization Mass Spectrometry Analysis [J].

Deng, Lu ;

Kitova, Elena N. ;

Klassen, John S. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2013, 24 (01) :49-56

[10] Optical tweezers: the next generation [J].

Dholakia, K ;

Spalding, G ;

MacDonald, M .

PHYSICS WORLD, 2002, 15 (10) :31-+

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