C/EBPα gene as a genetic marker for beef quality improvement

被引:9
作者
Adoligbe, C. [1 ]
Huangfu, Y. F. [1 ]
Zan, L. S. [1 ,2 ]
Wang, H. [1 ]
机构
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China
[2] Northwest A&F Univ, Natl Beef Cattle Improvement Ctr, Yangling, Shaanxi, Peoples R China
关键词
C/EBP alpha gene; Bovine muscle stem cells; Over-expression; Adipocyte differentiation; SATELLITE CELLS; ADIPOCYTE DIFFERENTIATION; ADIPOSE CONVERSION; EXPRESSION; ADIPOGENESIS; ADIPOBLASTS; PROTEINS; FAMILY; BETA;
D O I
10.4238/2015.August.14.1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Intramuscular fat (IMF) or intramuscular triglycerides are interspersed throughout the skeletal muscles. The IMF, also called marbling, imparts meat with flavor and juiciness and is one of the core criteria for judging carcass value. The quantity of IMF is influenced entirely by genetics. Recently, understanding the underlying genetic bases of IMF has been a focus particularly in the beef industry. In this study, with the deep insights of ameliorating the beef quality by genetic means, the role of the CCAAT/enhancer binding protein alpha (C/EBP alpha) gene was investigated by over-expressing C/EBP alpha in bovine muscle stem cells (MSCs) to initiate the adipogenic program. Prior to this, bovine MSCs were isolated and induced to differentiate into adipocytes from cells that were exposed to dexamethasone isobutylmethylxanthine and indomethacin; the presence of insulin and fetal bovine serum was examined. Either ectopic expression of C/EBP alpha or treatment with dexamethasone and insulin induced the accumulation of fat droplets and the expression of adipogenic induction genes (LPL, PPAR gamma, C/EBP beta, and C/EBP delta). The expression levels of myoblast-related genes (MyoD, Myf5, and Pax7) were also measured to assess the accuracy of the differentiation process. This study provides evidence that the C/EBP alpha gene is essential for cattle adipose tissue growth and development. Hence, this finding can contribute to improving beef carcass quality.
引用
收藏
页码:9370 / 9383
页数:14
相关论文
共 20 条
  • [1] Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal muscle satellite cells
    Beauchamp, JR
    Heslop, L
    Yu, DSW
    Tajbakhsh, S
    Kelly, RG
    Wernig, A
    Buckingham, ME
    Partridge, TA
    Zammit, PS
    [J]. JOURNAL OF CELL BIOLOGY, 2000, 151 (06) : 1221 - 1233
  • [2] REGULATED EXPRESSION OF 3 C/EBP ISOFORMS DURING ADIPOSE CONVERSION OF 3T3-L1 CELLS
    CAO, ZD
    UMEK, RM
    MCKNIGHT, SL
    [J]. GENES & DEVELOPMENT, 1991, 5 (09) : 1538 - 1552
  • [3] REGULATION OF ADIPOCYTE DEVELOPMENT
    CORNELIUS, P
    MACDOUGALD, OA
    LANE, MD
    [J]. ANNUAL REVIEW OF NUTRITION, 1994, 14 : 99 - 129
  • [4] Crameri R, 2005, J PHYSL, V565, P165
  • [5] RECIPROCAL REGULATION OF ADIPOGENESIS BY MYC AND C/EBP-ALPHA
    FREYTAG, SO
    GEDDES, TJ
    [J]. SCIENCE, 1992, 256 (5055) : 379 - 382
  • [6] SUBLINES OF MOUSE 3T3 CELLS THAT ACCUMULATE LIPID
    GREEN, H
    KEHINDE, O
    [J]. CELL, 1974, 1 (03) : 113 - 116
  • [7] ESTABLISHED PRE-ADIPOSE CELL LINE AND ITS DIFFERENTIATION IN CULTURE .2. FACTORS AFFECTING ADIPOSE CONVERSION
    GREEN, H
    KEHINDE, O
    [J]. CELL, 1975, 5 (01) : 19 - 27
  • [8] Trans-differentiation of myoblasts to adipoblasts: triggering effects of fatty acids and thiazolidinediones
    Grimaldi, PA
    Teboul, L
    Inadera, H
    Gaillard, D
    Amri, EZ
    [J]. PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1997, 57 (01): : 71 - 75
  • [9] Biological role of the CCAAT enhancer-binding protein family of transcription factors
    Lekstrom-Himes, J
    Xanthopoulos, KG
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (44) : 28545 - 28548
  • [10] CCAAT/ENHANCER BINDING-PROTEIN-ALPHA IS SUFFICIENT TO INITIATE THE 3T3-L1 ADIPOCYTE DIFFERENTIATION PROGRAM
    LIN, FT
    LANE, MD
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (19) : 8757 - 8761