Fluorometric and colorimetric dual-readout alkaline phosphatase activityassay based on enzymatically induced formation of colored Au@Ag nanoparticles and an inner filter effect

被引：31
作者：
Chen, Chuanxia [1 ]
Zhang, Guanglu [2 ]
Ni, Pengjuan [1 ]
Jiang, Yuanyuan [1 ]
Lu, Yizhong [1 ]
Lu, Zhengliang [3 ]
机构：
[1] Univ Jinan, Sch Mat Sci & Engn, Jinan 250022, Shandong, Peoples R China
[2] Shandong Normal Univ, Inst Mol & Nano Sci,Minist Educ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci,Key Lab Mol & Nano, Jinan 250014, Shandong, Peoples R China
[3] Univ Jinan, Sch Chem & Chem Engn, Key Lab Interfacial React & Sensing Anal Univ Sha, Jinan 250022, Shandong, Peoples R China
来源：
MICROCHIMICA ACTA | 2019年 / 186卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Ascorbic acid; Gold-silver nanoparticles; Graphene quantum dots; Fluorescence; Spectral overlap; Quencher; Pyrolysis; GRAPHENE QUANTUM DOTS; TIME FLUORESCENCE ASSAY; GOLD NANOPARTICLES; CARBON DOTS; NITROGEN; PROBE; PYROPHOSPHATE; IMMUNOASSAY; NANOSENSOR; SULFUR;
D O I：
10.1007/s00604-019-3478-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An ultrasensitive fluorometric and colorimetric dual-mode assay is described for the determination of the activity of alkaline phosphatase (ALP). ALP catalyzes the decomposition of 2-phospho-L-ascorbic acid, and the ascorbic acid thus generated reduces silver ions. In the presence of gold nanoparticles, gold-silver nanoparticles (Au@Ag NPs) are formed. This is accompanied by a color change form pink to deep yellow. The Au@Ag NPs reduce the fluorescence of blue fluorescent graphene quantum dots due to spectral overlap. The changes of absorbance (measured at 410 and 520nm) and fluorescence (measured at excitation/emission wavelengths of 346/415nm) correlate well with the ALP activity in the 0.01-6mUmL(-1) (absorption) and 0.01-2mUmL(-1) (fluorescence) ranges, and the detection limits are 9 and 5 UmL(-1) individually.
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页数：10
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