Antimicrobial resistance studies in staphylococci and streptococci isolated from cows with mastitis in Argentina

被引：9

作者：

Crespi, Elisa ^{[1
]}

Pereyra, Ana M. ^{[1
]}

Puigdevall, Tomas ^{[1
]}

Rumi, Maria, V ^{[1
]}

Testorelli, Maria F. ^{[1
]}

Caggiano, Nicolas ^{[2
]}

Gulone, Lucia ^{[3
]}

Mollerach, Marta ^{[3
]}

Gentilini, Elida R. ^{[1
]}

Srednik, Mariela E. ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Veterinarias, Catedra Microbiol, Av Chorroarin 280,C1427CWO, Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Ciencias Veterinarias, Catedra Fisiol Anim, Av Chorroarin 280,C1427CWO, Buenos Aires, DF, Argentina

[3] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol, Junin 954,C1113AAD, Buenos Aires, DF, Argentina

来源：

JOURNAL OF VETERINARY SCIENCE | 2022年 / 23卷 / 06期

关键词：

Bovine mastitis; antimicrobial resistance; staphylococci; streptococci; wmolecular typing; COAGULASE-NEGATIVE STAPHYLOCOCCI; METHICILLIN-RESISTANT; BOVINE MASTITIS; DAIRY-COWS; ANTIBIOTIC SUSCEPTIBILITY; SUBCLINICAL MASTITIS; CLINICAL MASTITIS; AUREUS; IDENTIFICATION; PREVALENCE;

D O I：

10.4142/jvs.21062

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: Staphylococcus aureus and Streptococcus agalactiae are the main cause of clinical mastitis in dairy cattle in Argentina, whereas coagulase-negative staphylococci (CNS) and environmental streptococci are the main cause of subclinical mastitis. Bacteria isolated from infected animals show increasing antimicrobial resistance. Objectives: This study aims to determine the antimicrobial resistance of staphylococci and streptococci isolated from milk with mastitis, and to genotypically characterize the methicillin-resistant (MR) staphylococci. Methods: Isolation was performed on blood agar and identification was based on biochemical reactions. Antimicrobial susceptibility was according to the Clinical and Laboratory Standards Institute guidelines. The antimicrobial resistance genes, SCCmec type and spa type were detected by the polymerase chain reaction method. Results: We isolated a total of 185 staphylococci and 28 streptococci from 148 milk samples. Among the staphylococcal isolates, 154 were identified as CNS and 31 as S. aureus. Among the 154 CNS, 24.6% (n = 38) were resistant to penicillin, 14.9% (n = 23) to erythromycin, 17.5% (n = 27) to clindamycin, 6.5% (n = 10) to cefoxitin and oxacillin. Among the S. aureus isolates, 16.1% (n = 5) were resistant to penicillin, 3.2% (n = 1) to cefoxitin and oxacillin (MRSA). Six MR isolates (5 CNS and 1 MRSA) were positive to the mecA gene, and presented the SCCmec IVa. The MRSA strain presented the sequence type 83 and the spa type 002. Among the 28 streptococcal isolates, 14.3% (n = 4) were resistant to penicillin, 10.7% (n = 3) to erythromycin and 14.3% (n = 4) to clindamycin. Conclusions: The present findings of this study indicate a development of antimicrobial resistance in main bacteria isolated from cows with mastitis in Argentina.

引用

页数：10

共 42 条

[41] Using whole-genome sequencing to determine appropriate streptomycin epidemiological cutoffs for Salmonella and Escherichia coli [J].