Determination of sugar conformation in large RNA oligonucleotides from analysis of dipole-dipole cross correlated relaxation by solution NMR spectroscopy

被引：25

作者：

Richter, C

Griesinger, C

Felli, I

Cole, PT

Varani, G

Schwalbe, H

机构：

[1] MIT, Dept Chem, Francis Bitter Natl Magnet Lab, Cambridge, MA 02139 USA

[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[3] Goethe Univ Frankfurt, Inst Organ Chem, D-60439 Frankfurt, Germany

来源：

JOURNAL OF BIOMOLECULAR NMR | 1999年 / 15卷 / 03期

关键词：

anisotropic tumbling; cross correlated relaxation; isotope labelling; ribose ring puckering; RNA;

D O I：

10.1023/A:1008319130714

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new experiment, the forward directed quantitative Gamma-HCCH-TOCSY for the measurement of the conformation of the five-membered ribosyl unit in RNA oligonucleotides, is presented. The experiment relies on quantification of cross peak intensities caused by evolution of CH,CH-dipole-dipole cross correlated relaxation in non-evolution periods and the resolution enhancement obtainable in forward directed HCC-TOCSY transfer. Cross correlated relaxation rates are interpreted to reveal the sugar conformation of 22 out of 25 nucleotides in an isotopically labelled 25-mer RNA. The results obtained with this new method are in agreement with the conformational analysis derived from (3)J(H,H) coupling constants.

引用

页码：241 / 250

页数：10

共 39 条

[21] Direct observation of asymmetric 1H/14N triplets and applications of asymmetric dipole-dipole splittings to structure determination by solid-state NMR spectroscopy
J Am Chem Soc, 29 (7805):
[22] DIRECT OBSERVATION OF ASYMMETRIC H-1/N-14 TRIPLETS AND APPLICATIONS OF ASYMMETRIC DIPOLE-DIPOLE SPLITTINGS TO STRUCTURE DETERMINATION BY SOLID-STATE NMR-SPECTROSCOPY
MCNAMARA, R
WU, CH
CHIRLIAN, LE
OPELLA, SJ
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (29) : 7805 - 7811
[23] C-13 AND H-1-NMR RELAXATION OF CHLOROFORM DISSOLVED IN A NEMATIC LIQUID-CRYSTAL .1. CROSS-CORRELATION BETWEEN INTRAMOLECULAR AND INTERMOLECULAR DIPOLE-DIPOLE INTERACTIONS
ISHIWATA, M
JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN, 1991, 60 (04) : 1379 - 1387
[24] NMR spectroscopic determination of angles α and δ in RNA from CH-dipolar coupling, P-CSA cross-correlated relaxation
Richter, C
Reif, B
Griesinger, C
Schwalbe, H
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (51) : 12728 - 12731
[25] Automated NMR determination of protein backbone dihedral angles from cross-correlated spin relaxation
Karin Kloiber
Wolfgang Schüler
Robert Konrat
Journal of Biomolecular NMR, 2002, 22 : 349 - 363
[26] Automated NMR determination of protein backbone dihedral angles from cross-correlated spin relaxation
Kloiber, K
Schüler, W
Konrat, R
JOURNAL OF BIOMOLECULAR NMR, 2002, 22 (04) : 349 - 363
[27] INTERFERENCE BETWEEN J-COUPLINGS AND CROSS-RELAXATION IN SOLUTION NMR-SPECTROSCOPY - CONSEQUENCES FOR MACROMOLECULAR STRUCTURE DETERMINATION
HARBISON, GS
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (07) : 3026 - 3027
[28] Determination of the solution conformation of a non-uniformly deuterium labelled (Uppsala 'NMR-window') 21mer RNA hairpin by NMR spectroscopy and computational methods.
Sandstrom, A
Maltseva, T
Chattopadhyaya, J
NUCLEOSIDES & NUCLEOTIDES, 1997, 16 (5-6): : 743 - 750
[29] Electron spin-lattice relaxation measurement of the 3Fe-4S (S-3) cluster in succinate:ubiquinone reductase from Paracoccus denitrificans.: A detailed analysis based on a dipole-dipole interaction model
Hung, SC
Grant, CV
Peloquin, JM
Waldeck, AR
Britt, RD
Chan, SI
JOURNAL OF PHYSICAL CHEMISTRY A, 2000, 104 (19): : 4402 - 4412
[30] Determination of the Glycosidic Bond Angle χ in RNA from Cross-Correlated Relaxation of CH Dipolar Coupling and N Chemical Shift Anisotropy
Schwalbe, H. (schwalbe@nmr.uni-frankfurt.de), 1962, American Chemical Society (126):

← 1 2 3 4 →