Characterization of Drosophila melanogaster cytochrome P450 genes

被引:230
作者
Chung, Henry [1 ]
Sztal, Tamar [1 ]
Pasricha, Shivani [1 ]
Sridhar, Mohan [1 ]
Batterham, Philip [1 ]
Daborn, Phillip J. [1 ]
机构
[1] Univ Melbourne, Bio21 Mol Sci & Biotechnol Inst, Dept Genet, Ctr Environm Stress & Adaptat Res, Melbourne, Vic 3010, Australia
基金
澳大利亚研究理事会;
关键词
detoxification genes; in situ hybridization; insecticide resistance; multigene family; RNAi; INTRON-EXON ORGANIZATION; INSECTICIDE RESISTANCE; TRANSCRIPTIONAL RESPONSE; BISEPOXIDE BIOSYNTHESIS; ECDYSONE BIOSYNTHESIS; MICROARRAY ANALYSIS; OMEGA-HYDROXYLASE; JUVENILE-HORMONE; GLOBAL ANALYSIS; P450; ENZYMES;
D O I
10.1073/pnas.0812141106
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cytochrome P450s form a large and diverse family of heme-containing proteins capable of carrying out many different enzymatic reactions. In both mammals and plants, some P450s are known to carry out reactions essential for processes such as hormone synthesis, while other P450s are involved in the detoxification of environmental compounds. In general, functions of insect P450s are less well understood. We characterized Drosophila melanogaster P450 expression patterns in embryos and 2 stages of third instar larvae. We identified numerous P450s expressed in the fat body, Malpighian (renal) tubules, and in distinct regions of the midgut, consistent with hypothesized roles in detoxification processes, and other P450s expressed in organs such as the gonads, corpora allata, oenocytes, hindgut, and brain. Combining expression pattern data with an RNA interference lethality screen of individual P450s, we identify candidate P450s essential for developmental processes and distinguish them from P450s with potential functions in detoxification.
引用
收藏
页码:5731 / 5736
页数:6
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