Single Cell Transfection with Single Molecule Resolution Using a Synthetic Nanopore

被引：17

作者：

Kurz, Volker ^{[1
]}

Tanaka, Tetsuya ^{[2
,3
]}

Timp, Gregory ^{[1
,3
]}

机构：

[1] Univ Notre Dame, Dept Elect Engn, Notre Dame, IN 46556 USA

[2] Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA

[3] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA

来源：

NANO LETTERS | 2014年 / 14卷 / 02期

基金：

美国国家科学基金会;

关键词：

Nanopore; electroporation; transfection; single molecule; single cell; ELECTROPORATION; EXPRESSION; SIMULATION; TRANSPORT; NOISE;

D O I：

10.1021/nl403789z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the development of a single cell gene delivery system based on electroporation using a synthetic nanopore, that is not only highly specific and very efficient but also transfects with single molecule resolution at low voltage (1 V) with minimal perturbation to the cell. Such a system can be used to control gene expression with unprecedented precision-no other method offers such capabilities.

引用

页码：604 / 611

页数：8