Single Cell Transfection with Single Molecule Resolution Using a Synthetic Nanopore

被引：17
|
作者：
Kurz, Volker [1 ]
Tanaka, Tetsuya [2 ,3 ]
Timp, Gregory [1 ,3 ]
机构：
[1] Univ Notre Dame, Dept Elect Engn, Notre Dame, IN 46556 USA
[2] Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA
[3] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA
来源：
NANO LETTERS | 2014年 / 14卷 / 02期
基金：
美国国家科学基金会;
关键词：
Nanopore; electroporation; transfection; single molecule; single cell; ELECTROPORATION; EXPRESSION; SIMULATION; TRANSPORT; NOISE;
D O I：
10.1021/nl403789z
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the development of a single cell gene delivery system based on electroporation using a synthetic nanopore, that is not only highly specific and very efficient but also transfects with single molecule resolution at low voltage (1 V) with minimal perturbation to the cell. Such a system can be used to control gene expression with unprecedented precision-no other method offers such capabilities.
引用
收藏
页码：604 / 611
页数：8
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