Fluorescent hydrophobic probes based on intramolecular charge transfer state for sensitive protein detection in solution

被引：14

作者：

Jun, H

Hong, SY

Yoon, SS ^{[1
]}

Kang, C

Suh, M

机构：

[1] Sungkyunkwan Univ, Dept Chem, Suwon 440746, South Korea

[2] Kyung Hee Univ, Dept Neurosci, Grad Sch E W Med Sch, Yongin 449701, South Korea

来源：

CHEMISTRY LETTERS | 2004年 / 33卷 / 06期

关键词：

D O I：

10.1246/cl.2004.690

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

New hydrophobic intramolecular charge transfer probes, comprising an aminostyryl and a cyanopyranyl moieties, were developed for sensitive protein quantification in solution. The probes exhibited dramatic increase of fluorescence quantum yields with accompanying blue-shift of the emission when bound to protein-sodium dodecyl sulfate (SDS) complexes. Using this emission enhancement, the synthesized probes were successfully applied for determining protein concentrations in solution.

引用

页码：690 / 691

页数：2

共 12 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] USE OF NILE RED AS A FLUORESCENT-PROBE FOR THE STUDY OF THE HYDROPHOBIC PROPERTIES OF PROTEIN SODIUM DODECYL-SULFATE COMPLEXES IN SOLUTION [J].