A new application of bioorganometallics:: the first simultaneous triple assay by the carbonylmetalloimmunoassay (CMIA) method

被引：49

作者：

Salmain, M

Vessières, A

Varenne, A

Brossier, P

Jaouen, G

机构：

[1] Ecole Natl Super Chim Paris, Lab Chim Organometall, UMR 7576, F-75231 Paris 05, France

[2] Fac Med & Pharm Dijon, Lab Microbiol Med, F-21033 Dijon, France

来源：

JOURNAL OF ORGANOMETALLIC CHEMISTRY | 1999年 / 589卷 / 01期

关键词：

metal carbonyl tracer; multi-immunoassay; Fourier transform IR spectroscopy; therapeutic drug monitoring;

D O I：

10.1016/S0022-328X(99)00358-7

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The first example of simultaneous triple carbonylmetalloimmunoassay (CMIA), a non-iso topic immunological method, is presented herein. This method, originally developed for mono-assays, uses various transition metal carbonyl complexes as labels and sensitive quantitation of these tracers by Fourier transform infrared (FT-IR) spectroscopy, thanks to the characteristic nu(CO) bands appearing in the 1800-2200 cm(-1) region. We show here that this method offers an advance in the challenging held of simultaneous multi-immunoassay, with the example of the assay of three antiepileptic drugs (carbamazepine, phenobarbital and diphenylhydantoin). (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：92 / 97

页数：6

共 25 条

[1] EFFECT OF BRIDGING LIGANDS ON C-O STRETCHING FORCE CONSTANTS OF COMPOUNDS CO2(CO)6(MU-Y)2 AND FE2(CO)6(MU-X)2 (WHERE Y = CO,P,AS,CR AND X = S,SR,SE,PRR',BR,I) [J].