Insulin acutely increases glucose transporter 1 on plasma membranes and glucose uptake in an AKT-dependent manner in chicken adipocytes

被引：11

作者：

Shimamoto, Saki ^{[1
,2
]}

Nakashima, Kazuki ^{[3
]}

Kamimura, Ryo ^{[1
]}

Kohrogi, Rukana ^{[1
]}

Inoue, Hiroki ^{[1
]}

Nishikoba, Nao ^{[1
]}

Ohtsuka, Akira ^{[1
,2
]}

Ijiri, Daichi ^{[1
,2
]}

机构：

[1] Kagoshima Univ, Dept Biochem Sci & Technol, 1-21-24 Korimoto, Kagoshima 8900065, Japan

[2] Kagoshima Univ, United Grad Sch Agr Sci, 1-21-24 Korimoto, Kagoshima 8900065, Japan

[3] NARO, Inst Livestock & Grassland Sci, Div Anim Metab & Nutr, 2 Ikenodai, Tsukuba, Ibaraki 3050901, Japan

来源：

GENERAL AND COMPARATIVE ENDOCRINOLOGY | 2019年 / 283卷

基金：

日本学术振兴会;

关键词：

Glucose transporter; AKT; Insulin; Adipocyte; Chicken; Aves; IN-VIVO; GLUT4; STIMULATION; EXPRESSION; TRANSPORT; METABOLISM; SKELETAL; MUSCLE; GENE;

D O I：

10.1016/j.ygcen.2019.113232

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Avian glucose transporters (GLUT) responsible for insulin-responsive glucose uptake into adipocytes remain poorly characterized. We aimed to identify the insulin-responsive GLUT using primary culture of chicken adipocytes. Acute stimulation with 1 mu M insulin for 20 min increased 2-deoxyglucose uptake, AKT protein phosphorylation, and GLUT1 protein levels on the plasma membrane of the chicken adipocytes, whereas pretreatment with 10 mu M triciribine, an AKT inhibitor, canceled these effects. Furthermore, the insulin stimulation did not affect GLUT12 protein levels on the plasma membrane of the chicken adipocytes. Our results suggest that GLUT1 is an insulin-responsive GLUT in chicken adipocytes.

引用

页数：6

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