Molecular Cytogenetics of Human Single Pronucleated Zygotes

被引：22

作者：

Azevedo, Ana Raquel ^{[1
]}

Pinho, Maria Joao ^{[2
]}

Silva, Joaquina ^{[3
]}

Sa, Rosalia ^{[1
]}

Thorsteinsdottir, Solveig ^{[4
,5
]}

Barros, Alberto ^{[2
,3
]}

Sousa, Mario ^{[1
]}

机构：

[1] Univ Porto, Inst Biomed Sci Abel Salazar ICBAS, Multidisciplinary Unit Biomed Res UMIB, Dept Microscopy,Lab Cell Biol, P-4050 Oporto, Portugal

[2] Univ Porto, Fac Med, Dept Genet, P-4050 Oporto, Portugal

[3] Ctr Reprod Genet, Oporto, Portugal

[4] Univ Lisbon, Dept Anim Biol, Fac Sci, Ctr Environm Biol, P-1699 Lisbon, Portugal

[5] Inst Gulbenkian Ciencias, Oeiras, Portugal

来源：

REPRODUCTIVE SCIENCES | 2014年 / 21卷 / 12期

关键词：

FISH; histone H3; human monopronucleated zygotes; immunocytochemistry; lamins; INTRACYTOPLASMIC SPERM INJECTION; PREIMPLANTATION GENETIC DIAGNOSIS; HUMAN OOCYTES; FERTILIZATION; EMBRYOS; TIME; CONSTITUTION; METHYLATION; PREGNANCY; CHROMATIN;

D O I：

10.1177/1933719114530185

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The aim of the present study was to use fluorescence in situ hybridization to analyze the chromosome status of zygotes with a single pronucleus from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment cycles. In addition, we performed immunocytochemical detection of nuclear lamins and histone H3 trimethylated at lysine-9, Me(3)H3K9. Zygotes were processed 24 hours after insemination or injection to assure the absence of asynchrony. In opposition to previous results, we observed 2 pronuclei in 16 of 18 IVF zygotes and 40 of 64 ICSI zygotes, suggesting premature pronuclear breakdown. In IVF and ICSI zygotes, the rate of normal diploidy was only 6 of 16 and 27 of 56, respectively, suggesting that monopronucleated zygotes should not be used in assisted reproductive treatments. The possible mechanisms are discussed and compared to previous studies of monopronucleated zygotes.

引用

页码：1472 / 1482

页数：11

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