Synthetic, site-specific biotinylated analogs of human MCP-1

被引：3
|
作者：
Kruszynski, Marian [1 ]
Tsui, Ping [1 ]
Stowell, Nicole [1 ]
Luo, Jinquan [1 ]
Nemeth, Jennifer F. [1 ]
Das, Anuk M. [1 ]
Sweet, Raymond [1 ]
Heavner, George A. [1 ]
机构：
[1] Centocor R&D Inc, Radnor, PA 19087 USA
来源：
JOURNAL OF PEPTIDE SCIENCE | 2006年 / 12卷 / 05期
关键词：
peptide synthesis; solid-phase peptide synthesis; MCP-1; CCL2; biotinylation; monoclonal antibodies (mAbs); calcium mobilization; receptor binding;
D O I：
10.1002/psc.734
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human monocyte chemoattractant protein 1 (MCP-1, CCL2) is a 8.6-kDa protein that has been implicated in a number of diseases including atherosclerosis, rheumatoid arthritis, chronic obstructive pulmonary disease and cancer. As part of a program to identify antibodies against MCP-1, we synthesized site-specific, biotinylated human MCP-1 analogs to be used for panning of an antibody phage display library. In contrast to material obtained from random biotinylation, the site-specific biotinylated analogs were homogeneous and retained full activity. Copyright (c) 2005 European Peptide Society and John Wiley & Sons, Ltd.
引用
收藏
页码：354 / 360
页数：7
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