Synthetic, site-specific biotinylated analogs of human MCP-1

被引：3

作者：

Kruszynski, Marian ^{[1
]}

Tsui, Ping ^{[1
]}

Stowell, Nicole ^{[1
]}

Luo, Jinquan ^{[1
]}

Nemeth, Jennifer F. ^{[1
]}

Das, Anuk M. ^{[1
]}

Sweet, Raymond ^{[1
]}

Heavner, George A. ^{[1
]}

机构：

[1] Centocor R&D Inc, Radnor, PA 19087 USA

来源：

JOURNAL OF PEPTIDE SCIENCE | 2006年 / 12卷 / 05期

关键词：

peptide synthesis; solid-phase peptide synthesis; MCP-1; CCL2; biotinylation; monoclonal antibodies (mAbs); calcium mobilization; receptor binding;

D O I：

10.1002/psc.734

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human monocyte chemoattractant protein 1 (MCP-1, CCL2) is a 8.6-kDa protein that has been implicated in a number of diseases including atherosclerosis, rheumatoid arthritis, chronic obstructive pulmonary disease and cancer. As part of a program to identify antibodies against MCP-1, we synthesized site-specific, biotinylated human MCP-1 analogs to be used for panning of an antibody phage display library. In contrast to material obtained from random biotinylation, the site-specific biotinylated analogs were homogeneous and retained full activity. Copyright (c) 2005 European Peptide Society and John Wiley & Sons, Ltd.

引用

页码：354 / 360

页数：7

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