Synthetic, site-specific biotinylated analogs of human MCP-1

被引：3
|
作者：
Kruszynski, Marian [1 ]
Tsui, Ping [1 ]
Stowell, Nicole [1 ]
Luo, Jinquan [1 ]
Nemeth, Jennifer F. [1 ]
Das, Anuk M. [1 ]
Sweet, Raymond [1 ]
Heavner, George A. [1 ]
机构：
[1] Centocor R&D Inc, Radnor, PA 19087 USA
来源：
JOURNAL OF PEPTIDE SCIENCE | 2006年 / 12卷 / 05期
关键词：
peptide synthesis; solid-phase peptide synthesis; MCP-1; CCL2; biotinylation; monoclonal antibodies (mAbs); calcium mobilization; receptor binding;
D O I：
10.1002/psc.734
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human monocyte chemoattractant protein 1 (MCP-1, CCL2) is a 8.6-kDa protein that has been implicated in a number of diseases including atherosclerosis, rheumatoid arthritis, chronic obstructive pulmonary disease and cancer. As part of a program to identify antibodies against MCP-1, we synthesized site-specific, biotinylated human MCP-1 analogs to be used for panning of an antibody phage display library. In contrast to material obtained from random biotinylation, the site-specific biotinylated analogs were homogeneous and retained full activity. Copyright (c) 2005 European Peptide Society and John Wiley & Sons, Ltd.
引用
收藏
页码：354 / 360
页数：7
相关论文
共 50 条
  • [1] Synthesis and biological characterization of human monocyte chemoattractant protein 1 (MCP-1) and its analogs
    Kruszynski, M
    Stowell, N
    Das, A
    Seideman, J
    Tsui, P
    Brigham-Burke, M
    Nemeth, JF
    Sweet, R
    Heavner, GA
    JOURNAL OF PEPTIDE SCIENCE, 2006, 12 (01) : 25 - 32
  • [2] SYNTHESIS AND CHARACTERIZATION OF SITE-SPECIFIC BIOTINYLATED PROBES FOR THE MOTILIN RECEPTOR
    MACIELAG, MJ
    PEETERS, T
    DEPOORTERE, I
    INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1994, 44 (06): : 582 - 588
  • [3] Repurposing site-specific recombinases for synthetic biology
    Cui, Lun
    St-Pierre, Francois
    Shearwin, Keith
    FUTURE MICROBIOLOGY, 2013, 8 (11) : 1361 - 1364
  • [4] Site-specific expression of IQGAP1 in human nephrons
    Wang, Ping
    Gong, Xiaojie
    Guan, Peizhong
    Ji, Dong
    Du, Linna
    Xu, Dongmei
    Liu, Yipeng
    JOURNAL OF MOLECULAR HISTOLOGY, 2019, 50 (02) : 119 - 127
  • [5] Site-specific expression of IQGAP1 in human nephrons
    Ping Wang
    Xiaojie Gong
    Peizhong Guan
    Dong Ji
    Linna Du
    Dongmei Xu
    Yipeng Liu
    Journal of Molecular Histology, 2019, 50 : 119 - 127
  • [6] SITE-SPECIFIC MUTAGENESIS OF HUMAN FOLLISTATIN
    INOUYE, S
    GUO, YL
    LING, N
    SHIMASAKI, S
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 179 (01) : 352 - 358
  • [7] Site-Specific Molecular Recognition of Proteins by Synthetic Receptors
    Urbach, Adam
    PROTEIN SCIENCE, 2014, 23 : 205 - 206
  • [8] Site-specific chromosomal integration of large synthetic constructs
    Kuhlman, Thomas E.
    Cox, Edward C.
    NUCLEIC ACIDS RESEARCH, 2010, 38 (06) : e92.1 - e92.10
  • [9] HUMAN MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1)
    LEONARD, EJ
    YOSHIMURA, T
    IMMUNOLOGY TODAY, 1990, 11 (03): : 97 - 101
  • [10] Site-specific incorporation of phenylalanine analogs into proteins in vivo.
    Wang, P
    Furter, R
    Son, S
    Tirrell, DA
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2002, 224 : U114 - U114
12345