Production of immunoregulatory polysaccharides from Crassostrea hongkongensis and their positive effects as a nutrition factor in modulating the effectiveness and toxicity of 5-FU chemotherapy in mice

被引:23
作者
Cai, Bingna [1 ]
Chen, Hua [1 ]
Sun, Han [2 ]
Wan, Peng [1 ]
Sun, Huili [1 ]
Pan, Jianyu [1 ]
机构
[1] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresource & Ecol, Guangdong Key Lab Marine Meteria Med, 164 Xingangxi Rd, Guangzhou 510301, Guangdong, Peoples R China
[2] Rutgers State Univ, 98 Brett Rd, Piscataway, NJ 08854 USA
基金
美国国家科学基金会;
关键词
IN-VITRO; IMMUNOMODULATORY ACTIVITY; T-CELLS; SULFATE; IMMUNONUTRITION; PROLIFERATION; SURGERY; DELTA; ASSAY; ACID;
D O I
10.1039/c5fo00885a
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chemotherapy is generally accompanied by undesirable side effects, such as immunosuppression and malnutrition, which reduce tolerance to cancer therapies. Prior studies have shown that immunonutrition improves the clinical outcomes of cancer patients. In this study, immunoregulatory polysaccharides from Crassostrea hongkongensis were included in a nutrition formula that was administered to S180 tumor-bearing mice in combination with 5-fluorouracil (5-FU) treatment. The C30-60% fraction of the polysaccharides was characterized as a branched polysaccharide, with a high amount of D-glucose (96.76% of the total) and the highest uronic acid and sulfate groups' content among all of the polysaccharide fractions. The C30-60% polysaccharide fraction showed a maximal proliferative effect on RAW264.7 cells and T lymphocytes at a concentration of 0.0391 mg mL(-1) and 0.0781 mg mL-1, respectively. Moreover, the combination treatment of the C30-60% polysaccharide- based nutrition formula (OPNF) with the administration of 5-FU effectively inhibited the growth of tumors and notably increased the leucocyte and lymphocyte counts in S180 tumor-bearing mice. In addition, a slight increase in the erythrocyte and hemoglobin values was observed in the mice treated with the combination of OPNF and 5-FU. These results suggest that supplementation with a C30-60%-based enteral formula would be beneficial for patients undergoing chemotherapy with 5-FU.
引用
收藏
页码:390 / 397
页数:8
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